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1、JournalofMicrobiologicalMethods65(2006)596–603www.elsevier.com/locate/jmicmethImmobilizationofhydrocarbon-oxidizingbacteriainpoly(vinylalcohol)cryogelshydrophobizedusingabiosurfactantMariaS.Kuyukinaa,*,IrenaB.Ivshinaa,AlexanderYu.Gavrina,ElenaA.Podorozhkob,bcdVladimirI.Lozinsky,ChrisE.Jeffree,Jam
2、esC.PhilpaInstituteofEcologyandGeneticsofMicroorganisms,UralBranchofRussianAcademyofSciences,13GolevStreet,614081,Perm,RussiabInstituteofOrganoelementCompounds,RussianAcademyofSciences,28VavilovStreet,119991,Moscow,RussiacElectronMicroscopeFacility,InstituteofMolecularPlantSciences,UniversityofEd
3、inburgh,TheKing’sBuildings,EdinburghEH93JH,Scotland,UKdSchoolofLifeSciences,NapierUniversity,10ColintonRoad,EdinburghEH105DT,Scotland,UKReceived26July2005;receivedinrevisedform7October2005;accepted13October2005Availableonline28November2005AbstractAsimplebiosurfactant-basedhydrophobizationprocedur
4、eforpoly(vinylalcohol)(PVA)cryogelswasdevelopedallowingeffectiveimmobilizationofhydrocarbon-oxidizingbacteria.TheresultingpartiallyhydrophobizedPVAcryogelgranules(granule3volume5Al)containedsufficientnumber(6.510)ofviablebacterialcellspergranule,possessedhighmechanicalstrengthandspontaneouslyloc
5、atedattheinterfaceinwater–hydrocarbonsystem.SuchinterfaciallocationofPVAgranulesallowedhighcontactofimmobilizedbiocatalystwithhydrophobicsubstrateandwaterphase,thusprovidingbacterialcellswithmineralandorganicnutrients.Asaresult,n-hexadecaneoxidationefficiencyof51%after10-dayincubationwasachievedu
6、singimmobilizedbiocatalyst.PVAcryogelswithincreasedhydrophobicitycanbeusedforimmobilizationofbacterialculturesperformingoxidativetransformationsofwater-immiscibleorganiccompounds.ImmobilizationofinsitubiosurfactantproducingRhodococcusbacteriaintoPVAcryogelisdiscussed.PVAcryogelgranuleswithentrapp
7、edalkanotrophicrhodococcalcellswerestableafter10-monthstorageatroomtemperature.D2005ElsevierB.V.Allrightsreserved.Keywords:Biosurfactant;Hydrophobization;Immobilizationcarrier;PVAcryogel;Rhodococcusbacteria1.Introducti
