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1、SUPPORTINGONLINEMATERIALContributionoffishtothemarineinorganiccarboncycle1*2*22,3452*R.W.Wilson,F.J.Millero,J.R.Taylor,P.J.Walsh,V.Christensen,S.Jennings&M.Grosell1SchoolofBiosciences,UniversityofExeter,EX44PS,UK.2RosenstielSchoolofMarine&AtmosphericSciences,UniversityofMiami,FL33149-109
2、8,USA.3UniversityofOttawa,Ottawa,Ontario,ONK196N5,Canada.4FisheriesCentre,UniversityofBritishColumbia,Vancouver,BritishColumbia,V6T1Z4,Canada.5CentreforEnvironment,FisheriesandAquacultureScience,Lowestoft,andSchoolofEnvironmentalSciences,UniversityofEastAnglia,Norwich,NR47JT,UK,*Towhomco
3、rrespondenceshouldbeaddressed.E-mail:r.w.wilson@ex.ac.uk(R.W.W.),fmillero@rsmas.miami.edu(F.J.M.),andmgrosell@rsmas.miami.edu(M.G.)1SUPPORTINGONLINEMATERIALMaterialsandMethodsTheChemicalNatureofFishCarbonatesWalshetal.(S1)reportedthatthecarbonatesfromtoadfish(Opsanusbeta)at25°Cresembledc
4、alciankutnorite[Ca0.74(Mg,Mn)0.26CO3]whenanalysedusingpowderX-raydiffraction.MorerecentlywehaveusedthesametechniqueoncarbonatesfromEuropeanflounder(Platichthysflesus)at13°Candfoundthatthesemostcloselyresemblethecrystalmake-upofmagnesiancalcite[Mg0.1Ca0.9CO3](Fig.S1;providedbyDr,RichardWa
5、lton,WarwickUniversity,UK).PowderX-raydiffractiondataweremeasuredusingaSiemensD5000diffractometeroperatingwithCuKaradiation.DatawererecordedfromfinelygroundpowderedsamplespressedintoaluminiumplatesinBragg-Brentanomodeandthepatternsmatchedagainstthe2000releaseoftheJCPDSpowderdiffractionfi
6、le.EstimationofGlobalFishBiomassandCarbonateProductionBasedonourlaboratorydataoncarbonateexcretionbyGulftoadfishandEuropeanflounder,togetherwiththewellestablishedrelationshipbetweenmetabolism,sizeandtemperature(S2),carbonateproductionratesforindividualfishfitarelationshipoftheform:8.025-
7、-4727(1/(T+273))Car.9809ì=10ew(EquationS1)2-1-1whereCiscarbonateproductionrate(mmolkgh),aandrareconstantsthatweresettoonetoachievethebestfittodataforfishthatwererestinginexperimentalchambers,wisindividualbodymass(g)andTistemperature(°C).Thisrelationshipisshowngraphicallyi
