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《Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、ARTICLEDOI:10.1038/s41467-017-02468-2OPENRedoxregulationofPEPactivityduringseedlingestablishmentinArabidopsisthalianaManuelGuineaDíaz1,2,TamaraHernández-Verdeja1,DmitryKremnev1,TimCrawford1,CaroleDubreuil1&?saStrand1Activationoftheplastid-encodedRNApolymeraseistightlycontrolledandinvol
2、vesanetworkofphosphorylationand,asyetunidenti?ed,thiol-mediatedevents.Here,wecharacterize1234567890PLASTIDREDOXINSENSITIVE2,aredox-regulatedproteinrequiredforfullPEP-driventran-scription.PRIN2dimerscanbereducedintotheactivemonomericformbythioredoxinsthroughreductionofadisul?debond.Expo
3、suretolightincreasestheratiobetweenthemonomericanddimericformsofPRIN2.Complementationofprin2-2withdifferentPRIN2proteinvariantsdemonstratesthatthemonomerisrequiredforlight-activatedPEP-dependenttranscriptionandthatexpressionofthenuclear-encodedphotosynthesisgenesislinkedtotheactivityof
4、PEP.ActivationofPEPduringchloroplastdevelopmentlikelyisthesourceofaretrogradesignalthatpromotesnuclearLHCBexpression.Thus,regulationofPRIN2isthethiol-mediatedmechanismrequiredforfullPEPactivity,withPRIN2monomerizationviareductionbyTRXsprovidingamechanisticlinkbetweenphotosyntheticelect
5、rontransportandactivationofphotosyntheticgeneexpression.1Ume?PlantScienceCentre,DepartmentofPlantPhysiology,Ume?University,SE-90187Ume?,Sweden.2Presentaddress:MolecularPlantBiology,UniversityofTurku,FI-20520Turku,Finland.ManuelGuineaDíazandTamaraHernández-Verdejacontributedequallytothi
6、swork.Correspondenceandrequestsformaterialsshouldbeaddressedto?.S.(email:Asa.Strand@umu.se)NATURECOMMUNICATIONS
7、(2018)9:50
8、DOI:10.1038/s41467-017-02468-2
9、www.nature.com/naturecommunications1ARTICLENATURECOMMUNICATIONS
10、DOI:10.1038/s41467-017-02468-2nadditiontobeingtheprimaryenergysource
11、forplants,allelesbothdemonstratedimpairedregulationofphotosynthesis-lightalsoprovidesplantswithinformationtocontrolassociatednucleargenes(PhANGs)whenexposedtoexcesslight,Iprocessessuchaschloroplastdevelopmentandseedlingorwhenphotosyntheticelectrontransport(PET)24wasinhibited.establis
