Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型

Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型

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Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型_第1頁
Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型_第2頁
Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型_第3頁
Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型_第4頁
Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型_第5頁
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《Culture-independent identification of gutbacteria correlated with the onset ofdiabetes in a rat model腸道文化獨(dú)立的鑒定 細(xì)菌與發(fā)病 糖尿病大鼠模型》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、TheISMEJournal(2009)3,536–548&2009InternationalSocietyforMicrobialEcologyAllrightsreserved1751-7362/09$32.00www.nature.com/ismejORIGINALARTICLECulture-independentidentificationofgutbacteriacorrelatedwiththeonsetofdiabetesinaratmodel11211LuizFWRoesch,Graciel

2、aLLorca,GeorgeCasella,AdrianaGiongo,AndresNaranjo,13143AriannaMPionzio,NanLi,VolkerMai,CliveHWasserfall,DesmondSchatz,431MarkAAtkinson,JosefNeuandEricWTriplett1DepartmentofMicrobiologyandCellScience,InstituteofFoodandAgriculturalSciences,Universityof2Florid

3、a,Gainesville,FL,USA;DepartmentofStatistics,UniversityofFlorida,Gainesville,FL,USA;34DepartmentofPediatrics,UniversityofFlorida,Gainesville,FL,USAandDepartmentofPathology,ImmunologyandLaboratoryMedicine,UniversityofFlorida,Gainesville,FL,USABacteriaassociat

4、edwiththeonsetoftype1diabetesinaratmodelsystemwereidentified.Intwoexperiments,stoolsampleswerecollectedatthreetimepointsafterbirthfrombio-breedingdiabetes-prone(BB-DP)andbio-breedingdiabetes-resistant(BB-DR)rats.DNAwasisolatedfromthesesamplesandthe16SrRNAge

5、newasamplifiedusinguniversalprimersets.Inthefirstexperiment,bandsspecifictoBB-DPandBB-DRgenotypeswereidentifiedbyautomatedribosomalintergenicspaceranalysisatthetimeofdiabetesonsetinBB-DP.LactobacillusandBacteroidesstrainswereidentifiedintheBB-DR-andBB-DP-sp

6、ecificbands,respectively.SangersequencingshowedthattheBB-DPandBB-DRbacterialcommunitiesdifferedsignificantlybuttoofewreadswereavailabletoidentifysignificantdifferencesatthegenusorspecieslevels.Asecondexperimentconfirmedtheseresultsusinghigherthroughputpyros

7、equencingandquantitativePCRof16SrRNAwithmoreratspergenotype.Anaverageof4541and338116SrRNAbacterialreadswereobtainedfromeachofthe10BB-DRand10BB-DPsamplescollectedattimeofdiabetesonset.NinegeneraweremoreabundantinBB-DPwhereasanotherninegeneraweremoreabundanti

8、nBB-DR.ThirteenandelevenspeciesweremoreabundantinBB-DPandBB-DR,respectively.Anaverageof23%and10%ofallreadscouldbeclassifiedatthegenusandspecieslevels,respectively.QuantitativePCRverifiedthehigherabunda

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