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1、TFFDevices切向流過濾裝置BasicConceptsofTangentialFlowFiltration(TFF)切向流過濾的基本概組件(TFF)MILLIPORE1Outline概要￠TypesofTFFDevices切向流過濾裝置的種類?openchannel開放型流道?turbulence-promotedchannels湍流增強型流道?"enhanced"bysecondaryflow次流道加強型￠Millipore'sdevicesandtheirperformance密理博公司TFF裝置及其性能￠RecommendedoperatingconditionsforMilli
2、pore'sTFFdevices密理博公司超濾裝置的建議操作條件MILLIPORE2TypesofTFFDevicesTFF裝置的類型Openchannel開放流道Flatplates(spacebetweenmembranes)平板(膜中間有空隙)Hollowfibersandtubes中空纖維及管Turbulence-promotedchannel湍流增強型流道Screenbetweenflatsheets/plates每層平板之間有篩網(wǎng)Spiral-wound(screenbetweenflatsheetswoundinaspiralpattern)卷式膜(卷起的膜每層之間有篩網(wǎng))MI
3、LLIPORE3TFFDeviceSelection--Considerations超濾裝置的選擇-從全面考慮的角度進(jìn)行￠Suspendedsolidsinfeed含懸濁的固狀物進(jìn)料?useopenchanneltopreventpluggingiftotalsuspendedsolids>2-5%byvolume?如果固狀懸濁物總量大于2-5%,使用開放流道型防止堵塞￠Availabilityofchosenmembrane可選用相應(yīng)的膜usuallyavailableinonly1or2devices通常只有一兩種超濾裝置可選￠Mechanicalcomplexity,reliabili
4、tyandease-of-use構(gòu)造復(fù)雜,可靠但使用簡單￠Simplicityofscale-up線形放大的簡單性￠Cost(deviceANDoperatingsystem)成本(設(shè)備本身及相應(yīng)操作系統(tǒng))MILLIPORE4Microporous(MF)TFFDevices切向流微濾裝置(MF)TFFMILLIPORE5TFFDevices,UsesandOperatingConditions切向流(微濾)裝置,使用及操作條件Microporous(MF)TFFDevices切向流微濾裝置(MF)?GlassReinforced(PES)flatplate(Prostak)改良聚醚砜外殼平
5、板膜(Prostak)–Flatplate,openflowchannel平板式,開放流道–(SIP)Steamableinplace(SIP)在線蒸汽滅菌–Minimumvolumes>5L–最小工作體積>5L?Applications:應(yīng)用–Cellharvestandclarification細(xì)胞收集和澄清–Bacterial,yeast,mammaliancells細(xì)菌,酵母,哺乳動物細(xì)胞–Lowshearapplications低剪切力應(yīng)用–Asepticapplications滅菌應(yīng)用MILLIPORE6Microporous(MF)TFFDevices–Prostak切向流微濾
6、裝置-ProstakPermeate透過Retentate回流MILLIPORE7TFFDevices,UsesandOperatingConditions切向流(微濾)裝置,使用及操作條件MicroporousTFFDevices切向流微濾裝置?Cassettes(Pellicon)盒式膜包(Pellicon)–Flatsheet,20-meshscreenedchannel平板式,20-MESH篩網(wǎng)–Notsteamable不能蒸汽滅菌–LowerminimumvolumesthanProstak比PROSTAK膜的殘留體積更小?Applications:應(yīng)用:Clarification
7、orbacterialcellharvest澄清或細(xì)菌,細(xì)胞收集-Whenminimumvolumesmustbe100mL-5L處理量范圍100mL-5LMaximumsuspendedsolids<5%toavoidplugging最大懸浮固狀物的量<5%避免堵塞孔道MILLIPORE8Microporous(MF)TFFDevices–Pellicon切向流微濾裝置-PelliconFeed進(jìn)口Perm
