Effect of microgrooved surface topography on osteoblast maturation and protein adsorption

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1、Effectofmicrogroovedsurfacetopographyonosteoblastmaturationandproteinadsorption12231AlbaC.DeLuca,MareikeZink,AstridWeidt,StefanG.Mayr,AthinaE.Markaki1DepartmentofEngineering,UniversityofCambridge,TrumpingtonStreet,CambridgeCB21PZ,UnitedKingdom2FacultyofPhysicsandEar

2、thSciences,InstituteforExperimentalPhysicsI,SoftMatterPhysicsDivision,UniversityofLeipzig,Linnestra?e5,04103Leipzig,Germany3LeibnizInstituteforSurfaceModi?cation(IOM),TranslationalCentreforRegenerativeMedicineandFacultyofPhysicsandEarthSciences,UniversityofLeipzig,

3、Permoserstra?e15,04318Leipzig,GermanyReceived28November2014;revised16January2015;accepted20January2015Publishedonline13February2015inWileyOnlineLibrary(wileyonlinelibrary.com).DOI:10.1002/jbm.a.35407Abstract:Microgroovedsurfaceshavebeenusedextensivelyafter28days.Toe

4、stablishwhetherpreferentialcellattach-toinfluencecellcontactguidance.Guidingcellgrowth,mentattheridge/grooveboundarieswasinfluencedbytheextracellularmatrixdeposition,andmineralizationisimpor-adhesionproteinscontainedintheserum-supplementedtantforboneimplantlongevity

5、.Inthisstudy,weinvestigatedmedia,fluorescentlylabeledfibronectinwasadsorbedontotheosteoblastresponsetomicrogroovedmetallicsurfacesinthemicrogroovedsubstratesatlowconcentrations,mimick-serum-supplementedmedium.Groovespacingwascompara-ingtheconcentrationsfoundinbloods

6、erum.Fibronectinblewiththespreadosteoblastsize.Focaladhesionswerewasfoundtoselectivelyadsorbontotheridge/grooveboun-observedtoconfinetotheinterveningridge/groovebounda-daries,theosteoblastfocaladhesionsites,suggestingthatries.Osteoblastsbridgedoverthegroovesandwereu

7、nableproteinadsorptionmayhaveinfluencedthecellattachmenttoconformtotheconcaveshapeoftheunderlyinggrooves.pattern.VC2015WileyPeriodicals,Inc.JBiomedMaterResPartA:Microgroovedsurfacesinducedhigherosteoblastprolifera-103A:26892700,2015.tionandmetabolicactivityafter14da

8、ysinosteogenicmediumcomparedwithas-receivedsurfaces,resultinginKeyWords:?bronectin,groovedsubstrates,osteoblastcells,highermineralizationa