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《2 The basic shape and fine structure of》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、2ThebasicshapeandfinestructureoforganellesObjectiveTofamiliarizewiththeshapeanddistributionoforganellesunderlightmicroscopeToknowtheprincipleandmethodsofdyeinglivingcellsTolearntodrawpicturesofspecimensundermicroscopematerialsLightmicroscope,glassslide,coverslip,lenspaperCedaroil,Xylene,Iodinesolu
2、tion,ObservethefollowingslidesundermicroscopePreparedslideofconvolutedtubuleofratkidney/線粒體Preparedcross-sectionofrabbitspinalganglion/高爾基體Preparedsectionofliver/肝糖procedure1MitochondriaObservepreparedslideofkidneyunder10*lens,youcanseetheroundconvolutedtubuleofkidneywithalayerofepithelialcells,ch
3、angeto40*lensandobservethedarkbluegranulesorshortbarsinthecytoplasmwhicharemitochondria.2golgicomplexObservethepreparedslideofrabbitspinalganglionunder10*lensandyoucanseetheyellowroundnervecells,changeto40*lens,therearemanyblackgranulesorirregularlongnarrowpiecesaroundthenucleuswhicharegolgibodies
4、.3glycogenObservethepreparedslideofliverwith10*lens,changeto40*lensandfindthereddotsinthelivercytoplasmwhichistheglycogen.It’stheenergystoreplaceinlivercell.SizeofOnionCellsPrepareawetmountoffreshonionandstainitwithIodinesolution.Trytogetthethinnestpiecethatyoucan;itshouldbethinnerthanpaper.Itmigh
5、thelpifyoutakeathickpieceandbendituntilitsnapsintotwopieces.Afteritsnaps,thereisusuallyaverythinconnectionremainingbetweenthetwopieces.Thisthinconnectinglayercanbepeeledoffandplacedonamicroscopeslideforviewing.Drawseveralonioncellsastheyappearunderhighpower.
