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1、J.gen.Virol.(1987),68,4756.PrintedinGreatBritain47Keywords:coronavirusMHV-JHM/nucleotidesequence/surfi~ceprojectionglycoproteingeneNucleotideSequenceoftheGeneEncodingtheSurfaceProjectionGlycoproteinofCoronavirusMHV-JHMByIRENESCHMIDT,MICHAELSKINNERS"ANDSTUARTSIDDELL*Inst
2、ituteofVirology,UniversityofWiirzburg,VersbacherStrasse7,8700Wiirzburg,F.R.G.(Accepted15September1986)SUMMARYSequencesencodingthesurfaceprojectionglycoproteinofthecoronavirus,murinehepatitisvirus(MHV),strainJHM,havebeenclonedintopAT153usingcDNAproducedbyprimingwithspeci
3、ficoligonucleotidesoninfectedcellRNA.TheregionsofthreeclonespJMS1010,pJS112andpJS92,whichtogetherencompassthesurfaceproteingenehavebeensequencedbythechainterminationmethod.Thesequenceoftheprimarytranslationproduct,deducedfromtheDNAsequence,predictsapolypeptideof1235amin
4、oacidswithamolecularweightof136600.Thispolypeptidedisplaysthefeaturescharacteristicofagroup1membraneprotein;anamino-terminalsignalsequenceandcarboxy-terminalmembraneandcytoplasmicdomains.Thereare21potentialglycosylationsitesinthepolypeptideandacysteine-richregioninthevi
5、cinityofthetransmembranedomain.Duringmaturationproteolyticprocessingofthepolypeptideoccursandatpositions624to628thesequenceArgArg-Ala-Arg-Argisfound,whichissimilartoanumberofbasicsequencesinvolvedinthecleavageofenvelopedRNAvirusglycoproteins.ThefusogenicpropertiesoftheM
6、HVsurfaceproteindonotappeartocorrelatewithastronglyhydrophobicregionattheputativeaminoterminusofthecarboxy-terminatcleavageproduct.INTRODUCTIONCoronavirusesarepleomorphic,envelopedviruseswhichreplicateinthecytoplasmofvertebratecellsandareassociatedwithdiseasesofeconomic
7、importance(Siddelletal.,1983a).Inthelaboratory,themurinehepatitisvirus(MHV)hasbeenextensivelyusedforthestudyofviralpathogenesis,inparticularasacomponentofamodelfordemyelinatingdiseasesofman(Knobleretal.,1982;Watanabeetal.,1983;Massaetal.,1986).TheMHVgenomeisamonopartite
8、,positive-strandedRNAofapproximately18kb.Thegenomeencodesthenucleocapsid(N),membrane(MorEl)andsurface(SorE2)pr
