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1、_ArchivesArchVirol(1995)140:t215--1223Vifrology?Springer-Verlag1995PrintedinAustriaGenomicrelationshipofporcinehemagglutinatingencephalomyelitisvirustobovinecoronavirusandhumancoronavirusOC43asstudiedbytheuseofbovinecoronavirusSgene-specificprobesE.Vieler,T.Schlapp
2、,C.Anders,andW.HerbstInstitutffirHygieneundInfektionskrankheitenderTierederJustus-Liebig-Universit/itGiessen,Giessen,FederalRepublicofGermanyAcceptedMarch2,1995Summary.Thegenomicrelationshipofporcinehemagglutinatingencephalomyelitisvirus(HEV)tobovinecoronavirus(BCV
3、)andhumancoronavirus(HCV)strainOC43wasexaminedbydotblothybridizationassays.TwoBCVSgene-specificprobesweregeneratedbypolymerasechainreactionfromtheavirulentLg-strainofBCV.ProbeswerelocatedintheS1andthe$2regionofthepeplomeric(S)glycoproteingene.TheS1probe(726bp)hybri
4、dizedwithBCVandHCV-OC43,butnotwithHEVundermoderatestringencyhybridizationconditions(50°C).Onlyslightsignalswerepresentwithmousehepatitisvirus(MHV)andnosignalswereobservedwithfelineinfectiousperitonitisvirus(FIPV)orcaninecoronavirus(CCV).Athighstringencyconditions(6
5、0°C)theS1probehybridizedwithBCVonly.Usingthe$2probe(680bp)undermoderatestrin-gencyconditions,hybridizationsignalswereobtainedwithBCV,HCV-OC43andHEV(strains67N,NT9,VW572).ThesignalsobtainedbythethreeHEVstrainswerealtogetherweakerthanwithBCVandHCV-OC43.The$2probedidn
6、otreactwithMHV,FIPVandCCV.AthighstringencytheS2-specificprobehybridizedwithBCVandHCV-OC43butdidnothybridizewithHEV.NucleotidesequenceanalysisoftheregioncoveringtheS2probeinHEVrevealed92.6%nucleotidesequencehomologytoBCVand91.9%toHCV-OC43.Incontrast,theregioncoverin
7、gtheS1probeinHEVcouldnotbeamplifiedusingtheBCVS1-specificprimers.ThehybridizationandsequencingresultsthusindicateaclosergenomicrelationshipbetweenBCVandHCV-OC43thanthereisbetweenHEVandBCVorHCV-OC43,respectively.IntroductionCoronavirusesareimportantpathogensinanimal
8、sandman.Twomajorantigenicgroupsofmammaliancoronavirusesaredistinguished.Porcinetransmissiblegastroenteritisvirus(TGEV),humanrespiratorycoronaviru
