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《冠狀病毒Coronavirusl論文-1995 Cloning, sequencing and expression of the S protein gene from two geographically distinct strains of canine coronav.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、VirusResearchELSEVIERVirusResearch39(1995)63-74Cloning,sequencingandexpressionoftheSproteingenefromtwogeographicallydistinctstrainsofcaninecoronavirus~BrianC.Horsburgh~,T.DavidK.Brown*DivisionofVirology,DepartmentofPathology,UniversityofCambridge,TennisCourtRoad,CambridgeCB21QP,UKReceived13March
2、1995;revisedandaccepted23June1995AbstractThegeneencodingthespike(S)proteinfromtwogeographicallydistinctstrains(AmericanandBritish)ofcaninecoronavirus(CCV)wasclonedandsequenced.Thenucleotidesequencerevealedopenreadingframesof1443or1453aminoacids,respec-tively.StructuralfeaturesincludeanN-terminal
3、hydrophobicsignalsequence,ahydrophiliccysteine-richclusterneartheC-terminus,twoheptadrepeatsand29or33potentialN-glycosylationsites.PairwisecomparisonsofSaminoacidsequencesfromtheseisolateswithotherCCVstrains(InsavclandK378)revealedthatheterogeneity,foundmostlyintheformofconservativesubstitutions
4、,isdistributedthroughoutthecaninesequences.However,5variableregionscouldbeidentified.Similaranalysiswithfeline,porcine,murine,chickenandhumancoronavirussequencesrevealedthatthecaninesequencesaremuchmorecloselyrelatedtothefelineSproteinsequencethantotheporcineSproteinsequenceseventhoughtheyareall
5、fromthesameantigenicgroup.Moreover,thesequencesimilaritybetweenCCVisolatesandthefelinecoronavirus,felineinfectiousperitonitisvirus(FIPV)wascomparable.ExpressionoftheCCVorthetransmissiblegastroenteritisvirus(TGEV)Sgeneusingthevacciniavirussystemproducedaproteinoftheexpectedsizewhichcouldinduceext
6、ensivesyncytiaformationininfectedcanineA72cells.Keywords:Coronavirus;Sprotein;SyncytiumThenucleotidesequencedatareportedinthispaperhavebeensubmittedtoGenBankandEMBL.*Correspondingauthor.Ipresentaddress:DepartmentofBiologicalChemistryandMolecularPharmacology,HarvardMedicalSchool,Boston,MA02115,US
7、A.0168-1702/95/$09.50?1995ElsevierScienceB.V.AllrightsreservedSSDI0168-1702(95)00068-264B.C.Horsburgh,T.D.K.Brown/VirusResearch39(1995)63-74Caninecoronavirus(CCV)belongstooneofthemajorantigenicgroupsofcoronaviruses(Siddellet
