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1、DNAANDCELLBIOLOGYVolume25,Number12,2006?MaryAnnLiebert,Inc.Pp.668–673BaculovirusSurfaceDisplayofSARSCoronavirus(SARS-CoV)SpikeProteinandImmunogenicityoftheDisplayedProteininMiceModelsQIANFENG,1YINGYINGLIU,1XIUXIAQU,1HONGKUIDENG,1MINGXIAODING,1TERENCEL.
2、T.LAU,2ALBERTCHEUNG-HOIYU,2andJIANGUOCHEN1ABSTRACTThebaculovirussurfacedisplaytechniquehasprovidedanidealtooltodisplayforeignproteinswithnaturalconformation,functions,andimmunogenicity.Inthiswork,weexploredtheapplicationofthistechniqueonSARS-associated
3、coronavirus(SARS-CoV)spike(S)protein,andfurtheranalyzedtheimmunogenicityofdis-playedSprotein.TheentireectodomainofSproteinwasfusedbetweenthegp64signalpeptideandtheVSV-Gmembraneanchorandsuccessfullydisplayedonthebaculovirussurface.Subcutaneousinjectionw
4、ithpurifiedS-displayedbaculoviruseswithoutadjuvantelicitedhighlyeffectiveproductionofspecificandneu-tralizingantibodiesagainstSproteininmice.TheseresultsconfirmedasuccessfulsurfacedisplayofSpro-teinonbaculoviruse,andsuggestedapotentialroleofS-displayed
5、baculovirusesasanovellivevirus-basedvaccinecandidateforSARS-CoV.INTRODUCTIONvectortoexpressheterologousproteinsininsectcellsandlar-vae(Kostetal.,2005).ThebaculovirussurfacedisplaysystemHESEVEREACUTERESPIRATORYSYNDROME(SARS),alsoisaspecializedbaculoviru
6、sexpressingsysteminwhichthetar-Tnamedinfectiousatypicalpneumonia,isanewlyemergentgetproteinisincorporatedintotheenvelopeofthebuddedtransmissiblefebrilerespiratorydisease.In2003,itcausedavirusesanddisplayedonthesurfaceofthevirions(Boubliketworldwideoutb
7、reakandinfectedmorethan8000peopleoveral.,1995;Grabherretal.,1997;Mottersheadetal.,1997).This26countries.ThecausativeagentofSARSwassoonidentifiedisaccomplishedbyfusingthetargetproteineithertotheC-ter-asanewmemberinthefamilyCoronaviridae(Ksiazeketal.,min
8、usofthegp64glycoproteinofAcMNPVorbetweenthe2003),andnamedsevereacuterespiratorysyndrome-associatedsignalpeptide(SP)andthetransmembranedomain(TM)ofthecoronavirus(SARS-CoV)bytheWorldHealthOrganizationgp64(Grabherretal.,1997).Inalternative
