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1、熒光素名稱(chēng)激發(fā)波長(zhǎng)(nm)發(fā)射波長(zhǎng)(nm)備注1,5IAEDANS336490?1,8-ANS372480?4-Methylumbelliferone385502?5-carboxy-2,7-dichlorofluorescein504529?5-Carboxyfluorescein(5-FAM)492518?5-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)54
2、2568?5-FAM(5-Carboxyfluorescein)492518?5-HAT(HydroxyTryptamine)370-415520-540?5-HydroxyTryptamine(HAT)370-415520-540?5-ROX(carboxy-X-rhodamine)578567604591?5-TAMRA(5-Carboxytetramethylrhodamine)548542552568?6-Carboxyrhodamine6G518543?6-CR6G518543?6-JOE5
3、20548?7-Amino-4-methylcoumarin351430?7-AminoactinomycinD(7-AAD)546647?7-Hydroxy-4-methylcoumarin360449,455?9-Amino-6-chloro-2-methoxyacridine412,430471,474?ABQ344445?AcidFuchsin540630?ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474?AcridineOrange
4、+DNA502526?AcridineOrange+RNA460650?AcridineOrange,bothDNA&RNA440-480520-650?AcridineRed455-600560-680?AcridineYellow470550?Acriflavin436520?AcriflavinFeulgenSITSA355-425460?Aequorin(Photoprotein)?466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiote
5、chnologies)seesgGFP,sgBFP???AlexaFluor350?346342442441?AlexaFluor430?431540?AlexaFluor488?495,492519,520?AlexaFluor532?531,532553,554?AlexaFluor546?556,557572,573?AlexaFluor568?577,578603?AlexaFluor594?590,594617,618?AlexaFluor633?632650?AlexaFluor647?6
6、47666?AlexaFluor660?668698?AlexaFluor680?679702?AlizarinComplexon530-560,580580,624-645?AlizarinRed530-560580?Allophycocyanin(APC)630,645655,660?AMC,AMCA-S345445?AMCA(Aminomethylcoumarin)345347425444?AMCA-X353442?AminoactinomycinD555655?Aminocoumarin346
7、350442445?Aminomethylcoumarin(AMCA)345347425444?AnilinBlue?600?Anthrocylstearate360-381446?APC(Allophycocyanin)630,645655,660?APC-Cy7625-650755?APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505?AstrazonBrilliantRed4G500585?AstrazonOrangeR4705
8、40?AstrazonRed6B520595?AstrazonYellow7GLL450480?Atabrine436490?ATTO-TAG?CBQCA465560?ATTO-TAG?FQ486591?Auramine460550?AurophosphineG450580?Aurophosphine450-490515?BAO9(Bisaminophenyloxadiazole)365395?BCECF(highpH)492,503520,528?BC
