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1、_Archives.......ArchVirol(1992)t25:39-52Virology?Springer-Verlag1992PrintedinAustriaResponsesofmicetomurinecoronavirusimmunizationA.L.Smith1'2,M.S.deSouza1'2,D.Finzi2,andS.W.Barthold11SectionofComparativeMedicineand2DepartmentofEpidemiologyandPublicHealt
2、h,YaleUniversitySchoolofMedicine,NewHaven,Connecticut,U.S.A.AcceptedDecember19,1991Summary.Oraland/orintranasalinoculationofsusceptiblemousegenotypeswiththeJHMstrainofmousehepatitisvirus(MHV-JHM)consistentlyresultsinTcelldysfunctionasreflectedbyinvitropr
3、oliferativeresponsestomitogensorallogeneiccells.OneapproachtoexaminingthemechanismresponsiblefortheobservedfunctionalTcellsuppressionistodeterminewhethervirusrep-licationisrequiredforitsinduction.Tothisend,micewereinoculatedoronasallywithMHV-JHMthatwasin
4、activatedwithshort-waveultravioletlight,beta-propiolactoneorpsoralen.MicewerealsoinoculatedwithliveMHV-JHMafterrecoveryfromhomotypicorheterotypicMHVinfection.SpleencellsfromBALBmiceinoculatedoronasallywithinactivatedMHV-JHMyieldedex-tremelyvariableinvitr
5、oproliferativeresponsesafterconcanavalinAstimu-lation.MHV-susceptiblemiceexposedoronasaUyorintraperitoneallytovirusinactivatedbyanyoftheminimumeffectivetreatmentsfailedtoseroconvert.Immunizationwithpsoralen-treatedvirusintraperitoneallyinFreund'scom-plet
6、eadjuvantororonasallyfailedtoprotectfromliveviruschallenge,butsurvivorshadelevatedvirus-specificserumIgGantibodytiterscomparedtomock-immunizedcontrolsattwoweekspost-challenge.Spleencellsfrommicethatwerechallengedafterrecoveryfromhomotypiclivevirusinfecti
7、ondidnotexhibittheprofoundinvitroTcellsuppressionnormallyobservedduringtheacutestageofprimaryinfection.Incontrast,MHV-JHMchallengeofmicevaccinatedwithheterotypicliveMHV-SresultedinsignificantlydepressedinvitroTceltfunction.Thecombineddatasuggestthateithe
8、rvirusreplicationorexposuretomoreconcentratedantigenmayberequiredforinductionofthedramaticTcelldysfunctionthatoccursasaconsequenceofMHV-JHMin-fectionaswellasforadetectableMHV-specifichumoralresponse.IntroductionMousehepati
