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557.Co-AdministrationofanAdenovirus558.Anti-SARSHumoralandCellularImmunityEncodingtheBCellStimulatingFactorBAFFwithEvokedbyanAdenovirusVectorExpressingSpikeHeat-InactivatedPseudomonasaeruginosaLeadsGlycoproteinfromSARSCoronavirustoIncreasedAnti-pseudomonalH
2、umoralImmunityJulieBoyer,1AnjaKrause,1JianpingQiu,1NeilHackett,1GaryChristineTertilt,1StefanWorgall,1MichaelC.Rivara,1RonaldG.Kobinger,2YanZhi,2JamesM.Wilson,2RonaldG.Crystal.111WeillMedicalCollegeofCornellUniversity,NewYork,NY;Crystal.12UniversityofPennsylvania,Philadelphia,PA.Weil
3、lMedicalCollegeofCornellUniversity,NewYork,NY.PseudomonasaeruginosaisanimportantpathogeninconditionsSevereacuterespiratorysyndrome(SARS)isanewlydescribedsuchassevereburnsandcysticfibrosis,causingsignificantmorbiditydiseasecausedbySARS-CoV,anovelcoronavirus.Duetothelimitedandmortalit
4、y.Despitemanyefforts,noclinicallyeffectivevaccinetreatmentoptionsandconcernoverconsequencesofSARSre-againstP.aeruginosaisavailabletodateespeciallyinCFpatients.emergence,wehavefocusedonusingadenovirus(Ad)-basedgeneInordertooptimizeanantipseudomonalvaccineandenhancetransfervectorsenco
5、dingSARS-CoVspecificantigenstodevelopmucosalimmunity,wefocusedourstudyontheenhancementoftheaneffectivevaccine.WetestedthehypothesisthatexpressionofBcellresponse.StimulatingBcellsthroughBcellactivatingmoleculestheSARS-CoVspikeglycoprotein(S),oritscomponentpartsS1duringimmunizationaga
6、instP.aeruginosamayincreasetheandS2,inE1-E3-Adserotype5vectorscanelicitcellularandefficiencyofavaccine.Bcellactivatingfactor(BAFF),a34kDhumoralimmuneresponsesagainstSinimmunizedmice.AsyntheticsinglechainTNF-familymember,isproducedbyactivatedantigenspikegenewasmadebyanoverlappingPCRs
7、trategywithcodonpresentingcellsandstimulatesBcells.Similartoothermembersofoptimizationformammaliancells.AnAdvectorexpressingSdriventheTNFfamily,BAFFissecretedasatrimerbycleavageofthebyaCMVpromoterwasconstructedandproteinexpressionwasproteinfromthemembrane.Followingbindingtoitsrecept
8、orsonBconfirmedbyWe
