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1、Evaluationofantibodiesagainstfelinecoronavirus7bproteinfordiagnosisoffelineinfectiousperitonitisincatsMelissaA.Kennedy,DVM,PhD;MohamedAbd-Eldaim,BVSc,MVSc,PhD;SarahE.Zika,DVM,MPH;JosephM.Mankin,DVM;StephenA.Kania,PhDObjective—Todeterminewhetherexpressionoffelinecorona
2、virus(FCoV)7bprotein,asindicatedbythepresenceofspecificserumantibodies,consistentlycorrelatedwithoccurrenceoffelineinfectiousperitonitis(FIP)incats.SamplePopulation—95serumsamplessubmittedforvariousdiagnosticassaysand20samplesfromspecific-pathogen–freecatstestedasnega
3、tivecontrolsamples.Procedures—The7bgenefromavirulentstrainofFCoVwasclonedintoaproteinexpres-sionvector.Theresultantrecombinantproteinwasproducedandusedinantibodydetec-tionassaysviawesternblotanalysisofserumsamples.Resultswerecomparedwiththoseofanimmunofluorescenceassa
4、y(IFA)forFCoV-specificantibodyandcorrelatedwithhealthstatus.Results—HealthyIFA-seronegativecatswereseronegativeforantibodiesagainstthe7bprotein.SomehealthycatswithdetectableFCoV-specificantibodiesasdeterminedviaIFAwereseronegativeforantibodiesagainstthe7bprotein.Serum
5、fromcatswithFIPhadantibodiesagainstthe7bprotein,includingcatswithnegativeresultsviaconventionalIFA.However,somehealthycats,aswellascatswithconditionsotherthanFIPthatweresero-positivetoFCoVviaIFA,werealsoseropositiveforthe7bprotein.ConclusionsandClinicalRelevance—Expre
6、ssionofthe7bprotein,asindicatedbydetectionofantibodiesagainsttheprotein,wasfoundinmostFCoV-infectedcats.SeropositivityforthisproteinwasnotspecificfortheFCoVvirulentbiotypeoradiagnosisofFIP.(AmJVetRes2008;69:1179–1182)elineinfectiousperitonitisisalethaldiseaseaffecting
7、FAbbreviAtionsdomesticandnondomesticfelidae.ItisasubstantialFCoVFelinecoronavirusproblemincatteries,multiple-cathouseholds,andFIPFelineinfectiousperitonitisshelters.ThediseasehasbeenestimatedtocostcatIFAImmunofluorescenceassaybreedersmorethan$2,000/catteryperyear.1Iti
8、salsoathreattomanyexoticfelidae,includingcheetahs,anendangeredspecies.2,3ThisdiseasecanoccurasTheseFIP-associatedlesionsrefl
