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1、第19卷第4期航天醫(yī)學與醫(yī)學工程Vo.l19No.42006年8月SpaceMedicine&MedicalEngineeringAug.2006ORIGNALRESEARCHEffectsofEmodinonExpressionofPneumonicCytokinesin*RatswithAcuteNecroticPancreatitisZHOUL-iming,CHAXue-xiang,WANL-ihong,ZHULing,KUANGXi
2、ang-hong,ZHOUXue-ping,JIANGJun-ming,WANGZheng-rong(DepartmentofBiomedicalEngineering,SchoolofBasicMedicineandForensicMedicine,WestChinaMedicalCen-ter,SichuanUniversity,Chengdu610041)Abstract:ObjectiveToinvestigatewhetheremodin(1,3,8-trihydroxy-6-methylant
3、hraquinone)canattenuateinflammatoryresponseinratslungswithacutenecroticpancreatitis(ANP).MethodA-cutenecroticpancreatitismodelwasinducedbyinjectionof3%sodiumtaurocholicacidintothesubcapsularofpancreasandemodinwasadministeredbyintestineperfusion.Plasmaamy
4、laseof3,6and12hwithacutenecroticpancreatitiswasmeasuredtogetherwiththedetectionofIL-1,IL-6andIL-10mRNAexpressionsinratslungsbysem-iquantitativeRT-PCR.ImmunohistochemistrywasdetectedtheexpressionofIL-1convertingenzyme(ICE)intheratslungs.ResultPlasmaamy
5、laseof3,6and12hwithacutenecroticpancreatitisgroupsareobviouslyhighascomparedwithnormalgroup(P<0.05).Plasmaamylasewas(1611.20218.72)IU/Linnormalgroup.Plas-maamylaseof3,6and12hwithacutenecroticpancreatitisgroupswere(1981.4056.81)IU/L,(3287.40612.37)IU/La
6、nd(4914.60746.82)IU/L.Plasmaamylaseof3,6and12hwithacutenecroticpancreatitisaftertreatmentwithemodingroupswereobviouslylowascomparedwitha-cutenecroticpancreatitisgroups.Theplasmaamylasewas(1617.20136.80)IU/L,(2323.40318.19)IU/Land(2670.20390.03)IU/Lres
7、pectively.ThestudyshowedthatthemRNAexpres-sionofpro-inflammatorycytokinIL-1andtheexpressionofIL-6,aswellastheexpressionofIL-1convertingenzyme(ICE)weredecreasedandIL-10wasincreased.ConclusionThestudydemon-stratesthatemodinplaysanmiportantroleinreducingpl
8、asmaamylaseleve.lEmodinexertsant-iin-flammatoryeffectsinacutenecroticpancreatitisratslungsbydownregulatingthemRNAexpres-sionofIL-1andIL-6andupregulatingthemRNAexpressionofIL-10.Keywords:emodin;acutenecroticpancrea
