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1、西北植物學(xué)報(bào),2010,30(11):2334-2339ActaBot.Boreal.Occident.Sin.文章編號(hào):10004025(2010)11233406不同產(chǎn)地土荊芥揮發(fā)油GC指紋圖譜研究1,21,2*1,2聶小妮,梁宗鎖,段琦梅(1西北農(nóng)林科技大學(xué)生命學(xué)院,陜西楊陵712100;2陜西省中藥指紋圖譜與天然產(chǎn)物庫研究中心,陜西楊陵712100)摘要:采用水蒸氣蒸餾法提取了12個(gè)產(chǎn)地土荊芥的揮發(fā)油,通過GC法分析其成分。運(yùn)用中藥色譜指紋圖譜相似度建立共有模式,以相關(guān)度
2、評(píng)價(jià)圖譜的相似性,以共有峰與對(duì)照峰的比值對(duì)各產(chǎn)地土荊芥藥材揮發(fā)油進(jìn)行聚類分析。結(jié)果表明:12個(gè)產(chǎn)地的土荊芥藥材的揮發(fā)油氣相色譜指紋圖譜有17個(gè)共有峰,其中氣相色譜指紋圖譜的相似度在0.85以上的有11個(gè),12個(gè)產(chǎn)地的土荊芥藥材的揮發(fā)油可通過系統(tǒng)聚類歸為兩類,不同產(chǎn)地土荊芥藥材的揮發(fā)油相似度較高。該方法簡(jiǎn)便,所得揮發(fā)油中各成分分離度高,建立的指紋圖譜重現(xiàn)性好,可作為土荊芥藥材揮發(fā)油的質(zhì)量控制方法之一。關(guān)鍵詞:土荊芥;產(chǎn)地;揮發(fā)油;氣相色譜;指紋圖譜中圖分類號(hào):Q503文獻(xiàn)標(biāo)識(shí)碼:AGCFinger
3、printofEssentialOilsofChenopodiumambrosioidesL.fromDifferentHabitats1,21,2*1,2NIEXiaoni,LIANGZongsuo,DUANQimei(1CollegeofLifeSciences,NorthwestAgriculture&ForestryUniversity,Yangling,Shaanxi712100,China;2ShaanxiResearchCenterinTraditionalChineseMe
4、dicineFingerprint&NaturalProductLibrary,Yangling,Shaanxi712100,China)Abstract:Steamdistillationmethodwasappliedtoextracttheessentialoilsof12habitatsfromChenopodiumambrosioidesL.TheessentialoilsweredeterminedwiththeGCmethod.BytheSimilarityEvaluationS
5、ystemforChromatographicFingerprintofTraditionalChineseMedicine,meanchromatogramwasgeneratedastherepresentativestandardfingerprintandthesimilarityofeachchromatogramagainstthemeanchromatogramwasalsocalculated.Sampleswasclusteredusingprincipalcomponent
6、analysis(PCA)basedontheratioofcharacteristicpeaksandstandardpeak.ThechromatographicfingerprintingofC.ambrosioidesL.,showing17characteristicpeaks,wasestablishedfrom12habitatsofC.ambrosioidesL.Thesimilarityofthechromatographicfingerprintsfrom11ofthe1
7、2sampleswasover0.85andthe12sampleswereclassifiedintotwogroupbasedonhierarchicalcluster,whichshowedthequalityofC.ambrosioidesL.fromdifferenthabitatswasingoodconsistency.ThemethodissimpleandtheessentialoilscanbeappliedforqualityassessmentofC.ambrosioid
8、esL.Keywords:ChenopodiumambrosioidesL.;habitats;essentialoils;gaschromatography(GC);chromatographicfingerprint土荊芥起源于熱帶美洲,現(xiàn)廣泛分布于世界熱nopodiumambrosioidesL.)帶有果穗的全草入藥,帶、亞熱帶及溫帶。以藜科藜屬植物土荊芥(Che始載于生草藥性備要,多作民間用藥。具有祛風(fēng)、收稿日期:20100622;修改稿收到日期:20100726基
