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1、中華移植雜志(電子版)2013年8月第7卷第3期ChinJTransplant(ElectronicEdition).August2013,Vo1.7,No.3·實(shí)驗(yàn)研究·不同灌注保存方法對(duì)豬離體供心保護(hù)作用的比較胡志斌嚴(yán)志妮王海濤潘曉華【摘要】目的比較不同灌注保存方式對(duì)離體豬心的保護(hù)作用。方法以36只健康雄性巴拿馬小香豬為實(shí)驗(yàn)對(duì)象,取出供心后根據(jù)保存技術(shù)不同分為不保存組、單純浸泡組和以50mL灌注容量為基礎(chǔ)并遞增,間隔時(shí)間為2h的50,100,150mL,200mL間斷灌注組,每組各6例。心臟灌注液和保存液均為二氮嗪強(qiáng)化的Celsi
2、or液(含二氮嗪30p~mol/L),保存溫度為4℃,保存時(shí)間10h。比較各組左心室心肌細(xì)胞凋亡指數(shù)(AI)、心肌組織ATP含量(ATP)、心肌含水量(MWC)和電鏡心肌超微結(jié)構(gòu)。結(jié)果心臟保存后,與不保存組比較,心肌細(xì)胞發(fā)生較多凋亡、心肌組織ATP明顯降低以及出現(xiàn)不同程度的心肌水腫損傷等。單純浸泡組AI最高(34.80±0.14)%,ATP水平最低(1.85±0.09)nmol/mg,MWC最高(82.03±0.11)%。200mL間斷灌注組AI最低(12.35±0.10)%,ATP水平最高(2.32±0.03)nmol/mg,MWC
3、最低(77.12-4-0.85)%;電鏡心肌超微結(jié)構(gòu)顯示,不保存組的心肌結(jié)構(gòu)最為正常,其次為150,200mL間斷灌注組。結(jié)論在長(zhǎng)時(shí)間心臟保存中,間斷灌注保存技術(shù)對(duì)心肌的保護(hù)作用優(yōu)于單純低溫浸泡保存技術(shù);間隔2h灌注1次,每次灌注容量為200mL的間斷灌注保存技術(shù)對(duì)心肌的保護(hù)效果較好?!娟P(guān)鍵詞】Clesior液;間斷灌注;豬;心臟;二氮嗪ComparativestudyofdiferentperfusionforprotectingheartgraftinpigsHUZhi—bin,YANZhi—kun。WANGttai—tao,PA
4、NXiao—hua.KeyLaboratoryofOrgansTransplantation,ZhqiangProvincialPeoplesHospital,HangZhou310014,ChinaCorrespondingauthor:HUZhi—bin,Email:hulinetl69@163.com【Abstract】0bjectiveTocomparetheprotectiveeffectsofdifferentperfusioninisolatedheartsofpigs.MethodsTheBamaminiaturepi
5、gsdonorheartswerearrestedwithCelsiorcardioplegiasolutionenhancedwithdiazoxide(DE,30p~mol/L).Isolatedheartswerenotpreservationinun—storedgroupandstoredinthesolutioninstacticcoldstoragegrouporplacedinadevicethatprovidingintermittentperfusionwith50,100,150,200mLsolutionres
6、pectivelyevery2hoursinintermittentperfusiongroupfor10hoursat4oC(n=6pergroup).Afterstorage,leftventriclesampleswerefrozen,extracted,thentheapoptoticindexofcardiomyocytes(AI),adenosinetriphosphateconcentrations(ATPr),myocardialwatercontent(MWC)andmyocardialuhrastructurewe
7、reanalyzed.ResultsAfterlong—timehypothermicstorage,comparedwithnn-storedgroup,thereweresignificantlyhigherwithAI,MWCandlowerwithATPcinthestacticcoldstoragegroupandintermittentperfusiongroup.ThestacticcoldstoragegroupshownthehighestAI[(34.80±0.14)%]andMWC[(82.03±0.11)%],
8、butlowestATPclevel[(1.85±0.09)].卟e200mLintermittentperfusiongroupshownthelowestAI[(12.35±0.10)%]andMWC[(77.12±