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1、256TianjinMedJ，March2015，Vo1．43No．3伐地那非對(duì)模擬高原低氧性肺動(dòng)脈高壓大鼠的作用周曉玲，潘磊，馬婷婷，郭蕊，王勇摘要：目的探討伐地那非防治高原低氧性肺動(dòng)脈高壓大鼠的效果及可能機(jī)制。方法將3O只大鼠隨機(jī)分為常壓常氧對(duì)照組(c組)、低壓低氧組(P組)和低壓低氧+伐地那非組(v組)，每組10只。采用低壓低氧艙模擬海拔5000m的高原環(huán)境(大氣壓50kPa，氧濃度10％)，P組和v組大鼠每日入艙8h，V組大鼠每日人艙前給伐地那非lmg／kg灌胃1次，C組和P組每日給予等劑量蒸餾水灌胃1次，共4周。4周后比較各組大鼠的平均肺動(dòng)脈壓、右心肥厚指數(shù)、肺血管
2、顯微形態(tài)學(xué)以及血清NO、內(nèi)皮素一I(ET一1)水平。結(jié)果P組的肺動(dòng)脈壓、右心肥厚指數(shù)、N4,動(dòng)脈管壁厚度占血管外徑的百分比(wT％)和管壁面積占血管總面積的百分比(wA％)顯著高于c組和V組(P<0．05)，血清NO低于c組和v組<0．05)，ET一1高于c組和v組(P<0．05)。結(jié)論伐地那非能降低高原肺動(dòng)脈高壓的肺動(dòng)脈壓力，減輕肺血管及右心重構(gòu)。關(guān)鍵詞：高血壓，肺性；磷酸二酯酶抑制劑；一氧化氮；內(nèi)皮縮血管肽1；高原性肺動(dòng)脈高壓；伐地那非中圖分類(lèi)號(hào)：R543．2文獻(xiàn)標(biāo)志碼：ADOI：10．3969~．issn．0253—9896．2015．03．009Theefectsof
3、vardenafilonhighaltitudepulmonaryhypertensioninratsZHOUXiaoling，PANLei，MATingting，GUORui，WANGYongDepartmentofGeratology,HypoxiaLaboratory,BeijingShiftanHospital，CapitalMedicalUniversity,Beijing100038，ChinaCorrespondingAuthorE—maihwangyongTO96@aliyun．cornAbstract：ObjectiveToinvestigatetheeff
4、ectofvardenafilonhighaltitudepulmonaryhypertensioninrats，andthepossiblemechanismthereof．MethodsThirtyratswererandomlydividedintothreegroups：controlgroupwithnormal——pres—sureandnormal—oxygen(groupC)，pulmonaryhypertensiongroupwithlow—pressureandlow—oxygen(groupP)，andthegrouptreatedbyvardenafi
5、linlow—pressureandlow—oxygencondition(groupV)．TheratsofgroupPandgroupVwereex—posedtolow——pressureandlow——oxygenconditioninanauto——modulatinghypobaricandhypoxiccabintosimulate5000mhighaltitudeenvironment(airpressure50kPa，oxygenconcentration10％)for8hoursdaily．Vardenafil(1mg／kg)wasgivenbygastr
6、ogavagetoratsingroupVoncedailyfor4weeks，whiletheisodosedistilledwaterwasgivenbygastrogavagetoratsingroupCandgroupP．Themeanpulmonaryarterialpressureandrightventricularmassindexweremeasuredrespectivelyafter4-weektreatment．Morphologicchangesofperipheralpulmonarya~eryweredetectedbylightmicrosco
7、pe．Theserumlevelsofnitricoxide(NO)andendothelin-1(ET一11weredetectedaswel1．ResultsThepulmonaryarterialpressureandrightventricularmassindexweresignificantlyhigheringroupPthanthoseofgroupCandgroupVfP<0．05)．Theratioofvascularmedialwallthicknesstoexternaldiam
