資源描述:
《采卵方法對(duì)西施舌早期發(fā)育生長(zhǎng)的影響-論文.pdf》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、特產(chǎn)研究SpecialWildEconomicAnimalandPlantResearch9文章編號(hào):1001—4721(2014)03—0009—04采卵方法對(duì)西施舌早期發(fā)育生長(zhǎng)的影響劉德經(jīng),朱善央(1.長(zhǎng)樂市漳港海蚌場(chǎng),福州350200;2.中國水產(chǎn)科學(xué)研究院東海水產(chǎn)研究所,上海20O090)摘要:采取自然排放(A組)、解剖取卵人工受精(B組)、陰干加流水刺激催產(chǎn)(C組)3種處理方法,將所獲得西施舌的受精卵發(fā)育成直線鉸合幼蟲,以微藻為餌料,在室內(nèi)條件下,幼蟲放養(yǎng)密度0.2ind/mL,經(jīng)9d~12d培養(yǎng),觀
2、察其發(fā)育生長(zhǎng)速度、成活率及變態(tài)率。試驗(yàn)結(jié)果表明,A組、B組、c組9日齡幼蟲平均殼長(zhǎng)272.00tan±18.04tma、239.60tma±19.97tma、260.00tma-i-20.07tan。日平均殼長(zhǎng)生長(zhǎng)速度21.11treed、17.51V-,n/d、19.78tma/d。平均殼長(zhǎng)A組與c組、C組與B組差異顯著(F=9.06,P<0.O1,n=30),A組、C組與B組差異極顯著(P<0.01);A組、B組、c組9日齡幼蟲成活率50.00%±4.82%、37.00%士6.64%、42.86%±3.57
3、%(F=6.00,P<0.05,n=3),A組與c組差異不顯著(P>0.05),A組、B組差異極顯著(P<0.01);A組、B組、c組幼蟲變態(tài)率51.33±6.05%、35.77±10.55%、40.59±3.19%(F=8.58,P<0.05,n=3),A組、B組差異極顯著(P<0.01),A組、c組差異顯著(P<0.05)。關(guān)鍵詞:西施舌;采卵方法;幼蟲發(fā)育生長(zhǎng)中圖分類號(hào):$966.22文獻(xiàn)標(biāo)識(shí)碼:AEfectsofOvnmMethodonEarlyGrowthandDevelopmentofCoeloma
4、ctraantiquateLIUDe—jing,ZHUShan—yang2(1.ChangleZhanggangSeaClamBreedingFarm,F(xiàn)uzhou350200,China;2.EastChinaSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Shanghai200090,China)Abstract:Takethenaturalemissions(A),Anatomyofthethreemethodsofoocyter
5、etrievalandartificialinsemination(B),edandwatertostimulateoxytocin(C).a(chǎn)ofstraighthingeweredevelopedforobtainingfertilizedeggsofCoelomactraantiquate.Usemicro—algaeasbaits,underlaboratoryconditions,larvalculturedensityof0.2ind/mLundertheconditionofcultureafter
6、9d一12d,guanobservedthedevelopmentoft~rowthrateandsurvivalrateandmetamorphosisrate.Resultsofexperiment,A,B,Cgroup9一day—oldlarvaeoftheaverageshelllengthwere272.0Own±18.04tan,239.60tma±19.97tma,260.00tma±20.07ttm,shellengthgrowthratewere21.11/m~/d,17.51t~n/d,19
7、.78/an/d.TherawlengIhdiferencesweresignificantforthedeterminationofresults.AandCandB,theaverageshellengthdiferenceof2.03timesand3,94times.Respectivelymistakenformachine,asignificantdiference.AnalysisvarianceF=9.06,(P<0.01,n=3O),Muld#ecompal'iflonsAandCandB,h
8、avealreadyreachedthe1%significanceleve1.(2)Respectively50.0o%±4.82%,37.00%±6.64%,42.86%±3.57%0fA,B,C9一day—oldlarvaesurvivalrate(%).AnalysisofvarianceF=6.00(P<0.05,n=3).diferencesignificant.Multi