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1、中回麈擘c童報2015,31(19):96-100ChineseAgriculturalScienceBulletin國槐花粉保存方法的初步研究牛燕娥,龐彩紅,李雙云2,3,夏陽,屈星(甘肅農(nóng)業(yè)大學,蘭州730000:山東省林業(yè)科學研究院,濟南250014;’山東省林木遺傳改良重點實驗室,濟南250014)摘要:研究旨在對國槐花粉進行提早采集和保存,避免人工輔助授粉和雜交授粉帶來的不便,延長花粉授粉期間的生活力。以國槐花粉為實驗材料,使用培養(yǎng)基萌發(fā)法觀察花粉萌發(fā)情況,測定花粉萌發(fā)所需時間,找出適于花粉萌發(fā)的培養(yǎng)基配方,同時比較
2、花粉在宣溫(27℃左右)和冰箱冷藏室(4℃)2種保存方法下的壽命。結果表明:6種培養(yǎng)基中最適于花粉萌發(fā)的培養(yǎng)基為1g瓊脂+1g蔗糖+100mg/L~t;花粉的萌發(fā)時間為萌發(fā)60rain;保存相同天數(shù)的情況下,冰箱冷藏室花粉萌發(fā)率明顯高于室溫萌發(fā)率,冰箱冷藏室保存5天的花粉可用于國槐授粉,此時的花粉萌發(fā)率大于50%。實驗獲得了適于國槐花粉進行野外授粉的保存方法,旨在為研究槐屬植物的雜交育種提供參考依據(jù)。關鍵詞:國槐;花粉;培養(yǎng)基;萌發(fā)率;保存方法中圖分類號:$792.26文獻標志碼:A論文編號:casb15010143APrel
3、iminaryStudyonSophorajaponicaPoBenPreservationMethodNiuYane,PangCaihong,LiShuangyun",XiaYang2,QuXing(‘GansuAgriculturalUni,~rsity,Lanzhou730000;2ShandongAcademyofForestry,Jinan250014;3ShandongProvinceKeyLaboratoryofForestTreeGeneticImprovement,Jinan250014)Abstract:T
4、hestudyaimsatearlyacquisitionandpreservationofSophorajaponicapollen,avoidinginconvenienceofartificialpollinationandcrosspollination,andprolongingthepollenviabilityduringpollenpollination.Simplemethodstoevaluatetheviabilityandtofindoutthetimeofpollengerminationwerees
5、tablished.Pollenviabilitywasassessedbyimprovingagrowingmediaandevaluatingthreecommonpollengerminationmediacomponents:agar,sucrose,andboricacid.Meanwhile,thepoHeninroomtemperature(about27℃)andintherefrigeratorfreezer(4a(=)werediferentiatedtodeterminetheirlongevity.Re
6、sultsshowedthatoptimalgerminationofpollenwasobtainedat1gagar+lgsucrose+lO0ms/Lboricacid.Thetimeofpollengerminationwas60minutes.UnderthesaInenumberofpreservationdays,thepolengerminationrateinrefrigeratorfreezerwassignificantlyhigherthanthatinroomtemperature.Thepollen
7、stored5daysinrefrigeratorfreezerwassuitableforpollenpollinationofSophorajaponica,andthepollengerminationratewasgreaterthan50percent.ThesuitablepreservationmethodforfieldpollinationofSophorajaponicapoUenhasbeenobtained,whichCanprovideareferenceforresearchonhybridizat
8、ionbreedingofSophoraplant.Keywords:Sophorajaponica;pollen;culturemedium;germinationrate;preservationmethodO引言科蝶形花亞科槐屬(SophoraL.)落葉喬木,在園林中適