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《S-腺苷蛋氨酸對(duì)大鼠肝臟部分缺血再灌注損傷的保護(hù)作用及機(jī)制研究-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、南通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)·366·JournalofNantongUniversity(Medica1Sciences)2013：33(5S一腺苷蛋氨酸對(duì)大鼠肝臟部分缺血再灌注損傷的保護(hù)作用及機(jī)制研究陳杰，邵偉斌，嚴(yán)棟梁，朱斌(南通大學(xué)第二附屬醫(yī)院肝膽外科，南通226001)[摘要】目的：探討S一腺苷蛋氨酸對(duì)大鼠肝臟部分缺血再灌注損傷的保護(hù)作用及機(jī)制。方法：將36只雌性SD大鼠隨機(jī)分為3gl(n=12)：假手術(shù)組、缺血再灌注組、S一腺苷蛋氨酸組。建立大鼠肝臟70％缺血再灌注模型，缺血時(shí)間為lh。腺苷蛋氨酸于術(shù)前1d及術(shù)后1h腹腔注射(100mg，k，假手
2、術(shù)組及缺血再灌注組在相同時(shí)間注射等量生理鹽水。各組大鼠分別于肝臟再灌注后6h和24h處死，取肝臟及血液標(biāo)本，檢測(cè)血清谷丙轉(zhuǎn)氨酶(alanineaminotransferase，AL、谷草轉(zhuǎn)氨酶(aspertateaminotransferase，AsD、腫瘤壞死因子一a(tumornecrosisfactord，TNF一，以及肝臟組織丙二醛(malonyldia1dehyde，MDA)含量，并觀察肝臟組織的病理改變。結(jié)果：與缺血再灌注組相比，S一腺苷蛋氨酸組的ALT、AST、TNF—ot水平明顯降低(P<0．01)，MDA含量明顯減少(P<0．05)，
3、肝臟病理損傷較輕。結(jié)論：S-腺苷蛋氨酸對(duì)大鼠肝臟缺血再灌注損傷具有保護(hù)作用．其機(jī)制可能與抗氧化及抑制炎癥因子產(chǎn)生和活化有關(guān)[關(guān)鍵詞]肝臟缺血再灌注損傷；S一腺苷蛋氨酸；丙二醛；腫瘤壞死因子一0【；大鼠[中圖分類號(hào)]R657【文獻(xiàn)標(biāo)志碼]A【文章編號(hào)]1674—7887(2013}05—0366—03TheprotectiveefectandmechanismofS-ademetionineonhepaticpartialischemia-reperfusioninjuryinratsCHEN船，SHA0Weibin，YANDongliang，ZHUBi
4、n(DepartmentofHepatobiliarySurgery，theSecondAfiliatedHospitalofNantongUniversity，Nantong226001)[Abstract]Objective：ToinvestigatetheprotectiveefectandmechanismofS-ademetionineagainsthepaticischemia-reper-fusioninjuryinratsanditsmechanism．Methods：Thirty-sixSDfemaleratswererandomly
5、dividedinto3groups，SHAM：shamoperationgroup，IR：ischemia—reperfusiongroup，SAMe：S-ademetioninetreatmentgroup．Themodelof70％hepaticischemia-reperfusionwasestablishedintherats．Ischemialastedforlh．TheratsofSAMegroupreceivedSAM(100mg／kg、intraperitoneally1dpriortooperationandlhafteropera
6、tion．Theothergroupsreceivedsalinesolutionatthesametime．Ratswerekilledat6，24hafterreperfusionrespectively．Bloodsampleswerecollectedandanalyzedforthelevelsofserum，atanineaminotransferase(ALT)，aspertateaminotransferase(AST)，tumornecrosisfactor0【(TNF—o【)．Themalonyldialdehyde(MDA)inl
7、ivertissuesweremeasuredandhistologicchangeswereobservedinHEstaining．Results：ComparisontoIRgroup，SAMegroupwasassociatedwithlowerserumtransaminasesALT，AST，TNF—o【．Besides，MDAexpressioninSAMegroupwassignificantlylowerthanthoseinIRgroup．TheinjuryoflivertissueinIRgroupwasmoreobviously
8、．Conclusion：S-ademetioninehasaprotectiveefectin
