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1、生三程食品,II斜枝Vo1.35,.No.09,2014轉(zhuǎn)谷氨酰胺酶對(duì)碎牛肉保水性和微觀結(jié)構(gòu)的影響董建國(guó)’,李茂華,潘潤(rùn)淑’,潘長(zhǎng)慶,馬瑞芬’,-B~gg(1.河南科技學(xué)院食品學(xué)院,河南新鄉(xiāng)453003;2.開(kāi)封市技師學(xué)院,河南開(kāi)封475000)摘要:為提高碎牛肉的利用率,將碎牛肉制成牛肉腸經(jīng)轉(zhuǎn)谷氨酰胺酶處理,在單因素實(shí)驗(yàn)的基礎(chǔ)上,通過(guò)響應(yīng)面分析得到轉(zhuǎn)谷氨酰胺酶降低牛肉腸蒸煮損失率的最適條件為酶量0_3%、反應(yīng)時(shí)間20rain、反應(yīng)溫度30℃,提高牛肉腸保水性的最適處理?xiàng)l件為酶量04%、反應(yīng)時(shí)間20min、反
2、應(yīng)溫度30℃,且經(jīng)該條件(酶量0.4%、反應(yīng)時(shí)間20min、反應(yīng)溫度30oC)處理后的牛肉腸各項(xiàng)指標(biāo)明顯優(yōu)于對(duì)照組,微觀結(jié)構(gòu)也更加規(guī)則有序。由此可知:適當(dāng)?shù)霓D(zhuǎn)谷氨酰胺酶處理可以有效改善碎牛肉的保水性能和微觀結(jié)構(gòu),具有較好的應(yīng)用前景。關(guān)鍵詞:轉(zhuǎn)谷氨酰胺酶.碎牛肉,響應(yīng)面,保水性,微觀結(jié)構(gòu)Effectsoftransglutaminaseonthewater-holdingcapacityandmicrOStructureofmincedbeefDONGJian—guo,LIMao—hua,PANRun—shu’
3、,PANChang—qing,MARui—fen,MAHan—jun(1.SchoolofFoodScience,HenanInstituteofScienceandTechnology,Xinxiang453003,China;2.KaifengInstituteofTechnology,Kaifeng475000,China)Abstract:lnordertomakefulIuseofIow—valuemincedbeef,theeffect0ftransglutaminasetreatmentonth
4、ebeefsausagebasedonmincedbeefwereinvestigated.Onthebaseofsinglefactorexperiments,responsesurfaceanalysiswasappliedtostudythefactorsofbeefsausage.Theresultsshowedthat:theoptimumconditionsforcookingloss0fmincedbeefweredeterminedas0.3%transglutaminaseconcentra
5、tion.2Ominreactiontimeand30oCreactiontemperature.TheoptimumconditionsforWater—holdingcapacityweredeterminedas0.4%transglutaminaseconcentration,20minreactiontimeand30℃reactiontemperature,andundertheseconditions,themajorindexesoftheobtainedproductcouldbesigni
6、ficantlyimproved.whosemicrostructurewasmoreregular.ItcouldbeconcludedthatappropriatetransglutaminasetreatmentwasapromisingtechnologysinceitcouldimprovethewaterretentionandmicrOstructureofmincedbeef.Keywords:transglutaminase;mincedbeef;responsesurfacemethodo
7、logy;water—holdingcapacity;microstructure中圖分類(lèi)號(hào):TS205文獻(xiàn)標(biāo)識(shí)碼:B文章編號(hào):1002~0306(2014)09—0145—05doi:10.13386/j.issnl002—0306.2014.09.023轉(zhuǎn)谷氨酰胺酶(TG)可以催化蛋白質(zhì)分子內(nèi)和體吸收利用?;谝陨显颍琓G在肉制品中的應(yīng)分子間發(fā)生交聯(lián)、蛋白質(zhì)和氨基酸之間連接、以及蛋用日益頻繁,但多數(shù)是關(guān)于魚(yú)糜、豬肉。、羊白質(zhì)分子內(nèi)谷氨酰胺基的水解,催化所形成的異肽肉、禽肉”的研究報(bào)道,對(duì)碎牛肉的影響與論
8、證鍵屬于共價(jià)鍵,在一般的非酶催化條件下很難斷裂,相對(duì)較少,缺乏嫻熟的工藝和有效的處理手段。因所以用該酶處理過(guò)的碎肉成型后雖經(jīng)冷凍、切片、烹此,在日常生產(chǎn)過(guò)程中,碎牛肉常常被作為低值肉被調(diào)也不會(huì)重新散開(kāi);此外,大量實(shí)驗(yàn)表明用谷氨酰胺進(jìn)行簡(jiǎn)單的出售,造成資源的浪費(fèi)和經(jīng)濟(jì)的損失。轉(zhuǎn)胺酶處理的食品對(duì)人是安全的,因?yàn)樗鼜V泛存在近幾年來(lái)隨著人flx,/牛肉的喜愛(ài)與重視程度的于人類(lèi)可食用的動(dòng)物組織,TG含有£一(