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《氮素營(yíng)養(yǎng)水平對(duì)衰老期煙葉氮代謝的影響及品種間差異.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、武云杰等氮素營(yíng)養(yǎng)水平對(duì)衰老期煙葉氮代謝的影響及品種間差異41氮素營(yíng)養(yǎng)水平對(duì)衰老期煙葉氮代謝的影響及品種間差異武云杰，李飛，楊鐵釗，張小全河南農(nóng)業(yè)大學(xué)煙草學(xué)院，鄭州I450002摘要：為揭示品種間煙葉品質(zhì)的差異和篩選耐氮肥品種，研究了烤煙品種在不同氮素營(yíng)養(yǎng)條件下氮代謝的差異。結(jié)果表明：氮低效與氮高效品種相比，可溶性蛋白和總氮降解量大，谷氨酰胺合成酶(GS)活性低，降幅大，谷氨酸脫氫酶(GDH)活性高，硝酸還原酶(NR)活性低，氨氣補(bǔ)償點(diǎn)高，品種間差異顯著。隨著施氮量的增加，氮低效品種氮素營(yíng)養(yǎng)物質(zhì)降解量百
2、分?jǐn)?shù)高，GS活性降幅大且受施氮量影響較小，GDH活性和氨氣揮發(fā)潛力增大，與氮高效品種相比差異顯著。NC89和豫煙10號(hào)分別在施氮量在45．o0和60．o0kg·hm。時(shí)氮代謝生理強(qiáng)度最大，而且豫煙10號(hào)各處理間差異小于NC89。說(shuō)明不同氮效率品種煙葉的氮素再同化和轉(zhuǎn)移能力存在差異，氮低效品種對(duì)氮素再同化量小，再轉(zhuǎn)移量大，對(duì)氮素的應(yīng)用范圍廣，調(diào)節(jié)能力強(qiáng)，而氮高效品種再同化量大，轉(zhuǎn)移量小，對(duì)氮素的應(yīng)用范圍小，調(diào)節(jié)能力弱。煙葉衰老期氮代謝與煙葉衰老速度有關(guān)，其中GS對(duì)NH的再同化能力和質(zhì)外體對(duì)NH的轉(zhuǎn)移能力
3、起主導(dǎo)作用。關(guān)鍵詞：烤煙；氮效率；氮代謝；氮素營(yíng)養(yǎng)doi：10．39690．issn．1004-5708．2014．04．009中圖分類號(hào)：$572．01文獻(xiàn)標(biāo)志碼：A文章編號(hào)：1004—5708(2014)04-0041-07EffectofnitrogennutritiononnitrogenmetabolisminageingtobaccoleafandvariationbetweenvarietiesWUYunjie，LIFei，YANGTiezhao，ZHANGXiaoquanCollage
4、ofTobacco，HenanAgriculturalUniversity，Zhengzhou450002，ChinaAbstract：Diferencesofnitrogen(N)metabolismunderdifferentnitrogennutritionwereinvestigatedtoselectnitrogen—resistanttobaccovarieties．Resultsshowedthat，comparedwithNeficientvariety,Nineficientvari
5、etyhadlargeamountofsolubleproteinandtotalNdegradation，lowglutaminesynthetase(Gs)andnitratereductase(NR)activity,highglutamatedehydrogenase(GDH)activityandammoniacompensationpoint．WiththeincreaseofNapplication，NineficientvarietYdisplayedhighpercentageofn
6、itrogennutrientdegradation，bigdecreaseofGSactivity,andincreasedGDHactivityandammoniavolatilizationpotentia1．NC89andYuyan10reachedthelargestNmetabolismphysicalstrengthattheNapplicationrateof45．00and60．00kg‘hm’andYuyan10showedlesschangesunderdiferenttreat
7、mentsasagainstNC89．ItwasconcludedthatvarietiesofdifferentNeficiencyonNassimilationandtransferabilitywerediferentforNineficientandNeficientvarieties，withtheformerboastinglargere—assimilationrate，smallamountofretransfer,widescopeofNapplicationandstrongadj
8、ustmentability．NmetabolismwasassociatedwithtobaccoleafagingspeedwithGSforNH4assimilationandapoplastforNH4transferplayingtheleadingrole．Keywords：flue—curedtobacco；nitrogeneficiency；nitrogenmetabolism；nitrogennutrition氮素是決定煙葉產(chǎn)量和質(zhì)量的
