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1、分類號(hào)S665學(xué)校代碼10129UDC635.6學(xué)號(hào)08202057不同處理方法對(duì)日本小果樹HASKAOP組培苗生根的影響EffectsofDifferentTreatmentMethodsonRootingofHASKAOP申請(qǐng)人:魏耐學(xué)科門類:農(nóng)學(xué)學(xué)科專業(yè):蔬菜學(xué)研究方向:園藝植物遺傳育種及生物技術(shù)指導(dǎo)教師:石嶺教授論文提交日期:二〇一一年六月摘要日本北海道特產(chǎn)小果樹HASKAOP(LoniceracaeruleaL.var.emphyllacaryxNakai)原為野生,屬于木本植物,具有較高的營(yíng)養(yǎng)價(jià)值
2、、藥用價(jià)值和經(jīng)濟(jì)價(jià)值。在組織培養(yǎng)中,芽容易誘導(dǎo)和增殖,根卻難以誘導(dǎo)。誘導(dǎo)生根是利用組織培養(yǎng)技術(shù)進(jìn)行微繁殖的關(guān)鍵。本試驗(yàn)對(duì)小果樹試管苗的生根處理方法及生根培養(yǎng)條件進(jìn)行了初步探索,主要研究結(jié)果如下:1.IBA對(duì)小果樹的生根有明顯促進(jìn)作用,NAA和IAA對(duì)其生根促進(jìn)作用不明顯。0.4mg/L的IBA是適宜小果樹生根的最佳濃度。2.在兩步生根過程中,適宜小果樹預(yù)處理生根誘導(dǎo)的IBA濃度是80mg/L,適宜小果樹預(yù)處理生根的IBA天數(shù)為11d。3.不同濃度IBA浸漬對(duì)小果樹生根有影響,最佳生根是在100mg/LIBA中
3、浸漬15min。4.適宜小果樹生根的基本培養(yǎng)基為1/2MS培養(yǎng)基。5.適合小果樹生根的暗培養(yǎng)時(shí)間為5d。6.適合小果樹生根的AC濃度為3g/L。7.適合小果樹生根的蔗糖濃度是20~30g/L。8.適合小果樹移栽的基質(zhì)為1/3蛭石+2/3田園土。關(guān)鍵詞:小果樹;不同處理方法;生根EffectsofDifferentTreatmentMethodsonRootingofHASKAOPAbstractTheJapaneseHokkaidospecialproductsmallfruittree(Loniceraca
4、eruleaL.var.emphyllacaryxNakai)isoriginallywildfruittree,andbelongswoodyplantwithhighnutritional,medicinalandeconomicvalues.Shootinductionandproliferationiseasytorootinducementoftissueculture.Rootingisthekeyofusingtissuecultureformicro-propagation.Thetesttu
5、beofsmallfruittreerootingandrootingtreatmentconditionswereexplored,themainresultsofthisresearchwereasfollows:1.IBAhadobviouseffectonthesmallfruittreesrooting.NAAandIAAwasnotobvious.Theoptimalconcentrationonrootingofsmallfruittreeswas0.4mg/LIBA.2.Inthetwo-st
6、epprocessofrooting,theoptimumIBAconcernmentforpretreatmentrootinginductionofsmallfruittreewas80mg/L.TheoptimumIBAtimeforpretreatmentrootingofSmallfruittreeswas11days.3.ThedifferentsucroseconcentrationsofIBAdippinghadeffectonrootingofsmallfruittree,theoptima
7、lconditiononrootingwasin100mg/LIBAdippingfor15min.4.Theoptimalbasicmediaforrootingofsmallfruittreewas1/2MSmedia.5.Theoptimaldarkculturetimeforrootingofsmallfruittreewas5d.6.TheoptimalACconcentrationforsmallfruittreerootingwas3g/L.7.Theoptimalsucroseconcentr
8、ationforrootingofsmallfruittreewasin20~30g/L.8.Theoptimalsubstratefortransplantingsmallfruittreewas1/3vermiculite+2/3gardensoil.Keywords:HASKAOP;Differenttreatment;RootingDirectedby:Prof.SHILing(Ph.D)A