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1、貴州農(nóng)業(yè)科學(xué)2014，42(9)：1O6～108GuizhouAgriculturalSciences[文章編號(hào)]1001—3601(2014)09—0533—0106—03PlantProtection·SoiIandFertilizer·Microoraanism杜仲灰斑病防治藥劑的篩選任明國(guó)，李榮玉。，劉紅昌，龍友華，吳小毛(1．貴陽(yáng)市開(kāi)陽(yáng)縣農(nóng)業(yè)局植保站，貴州開(kāi)陽(yáng)550025；2．貴州大學(xué)農(nóng)學(xué)院，貴州貴陽(yáng)550025；3．貴州省藥用植物繁育與種植重點(diǎn)實(shí)驗(yàn)室，貴州貴陽(yáng)550025)[摘要]為篩選出有效防治杜仲灰斑病的藥劑在生產(chǎn)上應(yīng)用，采用菌絲生
2、長(zhǎng)速率法，研究甲基托布津、百茵清、中生菌素、多抗霉素、多菌靈和苯醚甲環(huán)唑等6種殺菌劑對(duì)杜仲灰斑病菌的室內(nèi)毒力，并進(jìn)行了田間藥效試驗(yàn)。結(jié)果表明：室內(nèi)毒力測(cè)定，多抗霉素、多茵靈和苯醚甲環(huán)唑?qū)野卟【休^好的抑制作用，其EC5o分別為1．67／~g／mL、0．96tzg／mL和0．18~g／mL；而甲基托布津、百茵清和中生菌素對(duì)灰斑病菌的毒力相對(duì)較弱。經(jīng)田間藥效試驗(yàn)，多抗霉素1000倍液、多茵靈2000倍液和苯醚甲環(huán)唑2500倍液對(duì)杜仲灰斑病有較好的防治效果，第2次施藥10d后的防效分別可達(dá)74．36、8O．82和79．67，顯著優(yōu)于其他藥劑處理，可作為
3、防治杜仲灰斑病的首選藥劑。[關(guān)鍵詞]杜仲；灰斑??；殺菌劑；毒力測(cè)定；藥效；藥劑篩選[中圖分類(lèi)號(hào)]$436．611．1[文獻(xiàn)標(biāo)識(shí)碼]AScreeningofFungicidesControllingGrayLeafSpotDiseaseofEucommiaulmoidesRENMingguo，LIRongyu，LIUHongchang。，LONGYouhua，WUXiaomao(1．KaiyangPlantProtectionStationofAgriculturalBureau，Kaiyang，Guizhou550025；2．Agriculture
4、CollegeofGuizhouUniversity，Guiyang，Guizhou550025；3．GuizhouKeyLaboratoryofPropagationandCultivationonMedicinalPlants，Guiyang，Guizhou550025，China)Abstract：InordertoscreeneffectivefungicidescontrollinggraylearspotdiseaseofE．ulmoides，thetoxicityofsixfungicides(thiophanatemethyl，ch
5、lorotha1on訂，Zhongshengmycin，polyoxins，carbendazimanddifenoconazo1e)onE．ulmoidesgrayleafspotpathogenweredeterminedinlaboratoryusingmyceliumgrowthratemethod．ThefieldtrialofthesixfungicidescontrollinggraylearspotdiseaseofE．ulmoideswasalsocarriedoutunderthefieldcondition．Results：T
6、hiophanate—methyl，chlorothalonilandzhongshengmycinhadworseantimicrobialeffectonA．tenuis．polyoxin。carbendazimanddifenoconazo1ehadbetterantimicrobialeffectonA．tenuisthanothers，withEC50of1．67og／mL，0．96g／mLand0．18￡上g／mL，respectively．Thefieldtria1resultsalsoconfirmedthattendaysafte
7、rsecondsprayedapplication，thecontrolefficiencyofpolyoxin1000times，carbendazim2000timesanddifenoconazole2500timesreached74．36，8O．82and79．67，respectively．Controleficiencyofthethreeagentswassignificantlybetterthanthatofothertreatments．Therefore，polyoxin，carbendazimanddifenoconazo
8、lecouldberecommendedasthefirst—choicefungicidestocontrolgrayl
