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1、實(shí)驗(yàn)五Theexperimentaboutregulatoryfactorsonrespiratorymovement呼吸運(yùn)動(dòng)調(diào)節(jié)因素實(shí)驗(yàn)Rhythmicbreathingexerciseundernormalconditionsandadaptabilityofrespiratorymovementinavarietyofphysiologicalconditionchangesdependsontheregulationoftheneuralmechanism.正常情況下的節(jié)律性呼吸運(yùn)動(dòng)以及在各種生理狀態(tài)下呼吸運(yùn)動(dòng)所發(fā)生的適應(yīng)性改變都有賴(lài)于神經(jīng)機(jī)制的調(diào)節(jié)作用.Experimentalpr
2、inciplesThemedullaoblongataisthebasiccenterofregulatingrhythmicalrespiration.延髓是調(diào)節(jié)節(jié)律性呼吸的基本中樞。Reflexactivitieswhicharecausedbythestimulusfromrespiratorytract,chemicalchangesinlungsandblood,aretheimportantguaranteeforthenormalrhythmicbreathing.來(lái)自呼吸道、肺部及血液中化學(xué)成分改變的刺激所引起的反射活動(dòng)，是實(shí)現(xiàn)正常節(jié)律性呼吸的重要保證。Experimenta
3、lprinciplesExperimentalobjective●Learningmethodsofrecordingtherespiratorymovement學(xué)習(xí)呼吸運(yùn)動(dòng)的記錄方法●Toobservetheeffectofmorphineonrespirationandlearnhowtorescue觀察嗎啡對(duì)呼吸的影響及學(xué)習(xí)如何解救。Experimentalobjective●Toobservetheeffectsofnerve,bodyfluidfactorsonrespiratorymovement觀察神經(jīng)、體液對(duì)呼吸運(yùn)動(dòng)的影響Toverifytheregulationmechan
4、ismofrespiratorymovement驗(yàn)證呼吸運(yùn)動(dòng)的調(diào)節(jié)機(jī)制Totrainyourskillofhandlingchartpaper.訓(xùn)練處理圖紙的能力ExperimentalequipmentsBL-420E+biologicalsignalcollectingandprocessingsystem(BL-420E+生物機(jī)能實(shí)驗(yàn)系統(tǒng))endotrachealtube(氣管插管)、respirationtransducer(呼吸換能器)、injectors（注射器20ml、5ml)surgicalinstruments（手術(shù)器械）、50cmrubbertube（膠管）Experim
5、entalanimalExperimentaldrugs20%urethane(烏拉坦/氨基甲酸乙酯)、1%morphine嗎啡5%nikethamide尼可剎米naloxone納洛酮3%lacticacid乳酸溶液、CO2airpocket氣囊、N2airpocket氣囊Ⅰanesthesiaandfix麻醉固定1.Weigh稱(chēng)重Experimentalprocedure實(shí)驗(yàn)步驟2.Injecturathane（5ml/kg，ivandip.）耳源靜脈、腹腔注射20%烏拉坦慢3.fixtherabbit固定家兔（※maketheneckflat頸部平坦）Experimentalproced
6、ure1.Shearingoftheneck頸部剪毛2.Cutthecervicalskin4～5cmlongalongthemidline沿頸正中剪開(kāi)皮膚3.Separatebluntlythesubcutaneoustissueandmuscletoexposethetrachea,andputalineunderthetrachea鈍性分離皮下組織及肌肉，暴露氣管，穿一條線備用。4.Findcarotidsheathonbothsidesoftrachea,thenseparatethebilateralvagalN(thethickest)andputlinesunderthem在氣
7、管兩側(cè)找到頸動(dòng)脈鞘，并分離雙側(cè)迷走N(最粗的)，穿二條線備用。Ⅱ、operationsoftheneck頸部手術(shù)Ⅲ、trachealintubation氣管插管1/31/2氣管插管圖示2cmthyroidcartilage甲狀軟骨于甲狀軟骨下方2cm處的氣管軟骨之間切開(kāi)l/3～1/2氣管圓周，并向上開(kāi)倒T字型切口。用干棉球?qū)夤軆?nèi)血液及分泌物擦干凈，向肺方向插入氣管插管，并結(jié)扎。ⅣConnecttheexpe
