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1、Large-scaleAnimalCellCultures:DesignandOperationalConsiderationsK.RamasubramanyanandK.Venkatasubramanian1Dept.ofChemicalandBiochemicalEngineering,Rutgers,TheStateUniversityofNewJersey,P.O.Box909,Piscataway,NJ-08855USAListofAbbreviations....................................
2、........................141Introduction..................................................................142TheDesignChallenge...2.....................................................153CellularAspects..............................................................163.1Cell
3、Density..............................................................163.2CellularProductivity.......................................................163.3AttachmentCharacteristics.................................................173.4CellRigidity...........................
4、...................................183.5GeneticStability...........................................................184BioreactorAspects............................................................184.1TheCellSystem...........................................................
5、194.2ChemicalEngineeringAspects...............................................194.3MechanicalAspects........................................................204.4ReactorTypes............................................................204.5ModeofOperation...................
6、.....................................214.6Scale-up..................................................................225DownstreamProcessing........................................................235.1TheProduct..............................................................
7、235.2ProcessScheme............................................................235.3ProductPurityandConcentration............................................245.4Medium..................................................................246CellCultureMedium...................
8、......................................247Epilog.................................................
