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《structure and flexibility in cold-adapted iron superoxide dismutases the case》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在工程資料-天天文庫。
1、JournalofStructuralBiology172(2010)343–352ContentslistsavailableatScienceDirectJournalofStructuralBiologyjournalhomepage:www.elsevier.com/locate/yjsbiStructureand?exibilityincold-adaptedironsuperoxidedismutases:ThecaseoftheenzymeisolatedfromPseudoalteromonashaloplanktisa,baccAntonelloMerli
2、no,IreneRussoKrauss,ImmacolataCastellano,EmmanueleDeVendittis,aaa,ba,b,?BiancaRossi,MariaConte,AlessandroVergara,FilomenaSicaaDipartimentodiChimica,UniversitàdiNapoliFedericoII,ComplessoUniversitarioMonteS.Angelo,ViaCinthia,I-80126Naples,ItalybIstitutodiBiostruttureeBioimmagini,CNR,ViaMezz
3、ocannone16,I-80134Naples,ItalycDipartimentodiBiochimicaeBiotecnologieMediche,UniversitàdiNapoliFedericoII,ViaPansini5,I-80131Napoli,ItalyarticleinfoabstractArticlehistory:Superoxidedismutases(SODs)aremetalloenzymescatalysingthedismutationofsuperoxideanionReceived23April2010radicalsintomole
4、cularoxygenandhydrogenperoxide.Here,wepresentthecrystalstructureofaReceivedinrevisedform29July2010cold-adaptedFe-SODfromtheAntarcticeubacteriumPseudoalteromonashaloplanktis(PhSOD),andthatAccepted18August2010ofitscomplexwithsodiumazide.Thestructureswerecomparedwiththoseofthecorrespondinghom
5、o-Availableonline21August2010logueshavingahighsequenceidentitywithPhSOD,suchasthemesophilicSODfromEscherichiacoli(EcSOD)orPseudomonasovalis,andthepsychrophilicSODfromAliivibriosalmonicida(AsSOD).TheseKeywords:enzymessharedalargestructuralsimilarity,suchasaconservedtertiarystructureandarran
6、gementCrystalstructureofthetwomonomers,analmostidenticaltotalnumberofinter-andintramolecularhydrogenbondsCold-adaptedenzymesSuperoxidedismutaseandsaltbridges.However,thetwocold-adaptedSODsshowedanincreased?exibilityoftheactivesiteDynamicsresidueswithrespecttotheirmesophilichomologues.Struc
7、turalinformationwascombinedwithachar-ThermalstabilityacterisationofthechemicalandthermalstabilityperformedbyCDand?uorescencemeasurements.PsychrophilicproteinsDespiteofitspsychrophilicorigin,thedenaturationtemperatureofPhSODwascomparablewiththatofthemesophilicE
