放線菌發(fā)酵產(chǎn)物對(duì)核盤菌的抑制_畢業(yè)設(shè)計(jì)(doc畢業(yè)設(shè)計(jì)論文)

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1、畢業(yè)論文放線菌T111發(fā)酵液理化性質(zhì)及發(fā)酵產(chǎn)物對(duì)核盤菌抑菌機(jī)制初探目錄中文摘要4英文摘要41前言51.1生防菌應(yīng)用的種類61.2拮抗放線菌的主要作用機(jī)制71.3本研究的目的及意義82材料與方法82.1材料82.2拮抗放線菌發(fā)酵液抑菌活性測(cè)定方法92.3T111發(fā)酵液的理化性質(zhì)92.4放線菌T111發(fā)酵產(chǎn)物對(duì)核盤菌的抑菌機(jī)制研究103結(jié)果與分析113.1T111發(fā)酵液的理化性質(zhì)113.1.1發(fā)酵液抗菌譜113.1.2貯存穩(wěn)定性123.1.3熱穩(wěn)定性133.1.4酸堿穩(wěn)定性133.1.5紫外光穩(wěn)定性143.2T111發(fā)酵產(chǎn)物對(duì)核盤菌的抑菌機(jī)制結(jié)果153.2.1T111發(fā)酵產(chǎn)物對(duì)核盤菌菌絲生

2、長(zhǎng)的影響結(jié)果153.2.2T111發(fā)酵產(chǎn)物對(duì)核盤菌菌絲形態(tài)的影響結(jié)果153.2.3T111發(fā)酵產(chǎn)物對(duì)核盤菌菌核形成及單重的影響結(jié)果163.2.4T111發(fā)酵產(chǎn)物對(duì)核盤菌菌核萌發(fā)的影響結(jié)果173.2.5T111發(fā)酵產(chǎn)物對(duì)核盤菌菌絲體細(xì)胞膜滲透勢(shì)的影響結(jié)果184討論195參考文獻(xiàn)206致謝21CatalogueChineseabstract4Englishabstract41Introduction51.1Thecategoryofantagonismfungus61.2TheantagonisticmechanismsofStreptomyces71.3Purposeandsignifi

3、canceofthestudy82Materialandmethods82.1material82.2Antifungalabilitiesoffermentationconditions92.3PhysicalandchemicalpropertiesofT111strain92.4AntagonisticmechanismstoSclerotiniasclerotiorumofT111103Resultsandanalyse113.1PhysicalandchemicalpropertiesofT111strain113.1.1Inhibitionspectrumofferment

4、ationextractsofT11113.1.2Effectsofstoragetimeontheactivityoffermentationextracts123.1.3Effectsoftemperaturesontheactivityoffermentationextracts133.1.4EffectsofpHontheactivityoffermentationextracts133.1.5EffectsofUVirradiationtimeontheactivityoffermentationextracts143.2Theresultofantagonisticmech

5、anismstoSclerotiniasclerotiorum153.2.1InhibitiontoxicitiesonmyceliumgrowthofS.sclerotiorum153.2.2EffectoffermentationbrothofT111onsclerotiummyceliumshape153.2.3EffectofantifungalsubstanceofT111onsclerotiumformationtimeandsingleweight163.2.4EffectofantifungalsubstanceofT111onsclerotiumbourgeon173

6、.2.5EffectofantifungalsubstanceofT111onmembraneosmoticpotential184Discussion195Reference206Thanks21放線菌T111發(fā)酵液理化性質(zhì)及發(fā)酵產(chǎn)物對(duì)核盤菌抑菌機(jī)制初探作者:韓紅寧（06植檢一）指導(dǎo)教師：慕衛(wèi)摘要：為明確放線菌T111的理化性質(zhì)，研究了其抗菌譜，及貯存時(shí)間、溫度、酸堿條件、紫外光照射等對(duì)其發(fā)酵液穩(wěn)定性的影響。結(jié)果表明，T111發(fā)酵液對(duì)黃瓜菌核、黃瓜蔓枯、棉花紅腐病菌等有較高的抑制活性；貯存穩(wěn)定性和熱穩(wěn)定性良好，4℃貯存60d抑菌活性為對(duì)照的88.3%，80℃處理60min抑菌活性為對(duì)

7、照的90.9%；對(duì)pH值較敏感，在pH5～7時(shí)活性較高；對(duì)紫外線穩(wěn)定，紫外光照射12h，其活性基本不受影響。初步研究了T111發(fā)酵產(chǎn)物對(duì)核盤菌的抑菌機(jī)制，采用菌絲生長(zhǎng)速率法測(cè)定了其對(duì)黃瓜菌核病菌Sclerotiniasclerotiorum菌絲生長(zhǎng)的毒力，及其對(duì)菌絲形態(tài)、菌核形成、菌核萌發(fā)和菌體細(xì)胞膜透性的影響。結(jié)果表明，T111發(fā)酵產(chǎn)物對(duì)核盤菌菌絲生長(zhǎng)的EC50值為12.5μg/mL，低于對(duì)照藥劑乙霉威及嘧霉胺；經(jīng)發(fā)酵產(chǎn)物處理后，菌絲形態(tài)出現(xiàn)