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1、中國科技論文在線http://www.paper.edu.cn富氫鹽水對東莨菪堿誘導(dǎo)大鼠干眼模型眼#表保護(hù)作用**楚瑩瑩，華寧，茹玉莎，趙少貞5（天津醫(yī)科大學(xué)眼科醫(yī)院，天津醫(yī)科大學(xué)眼視光學(xué)院，天津醫(yī)科大學(xué)眼科研究所，天津，300384）摘要：目的探討富氫鹽水(HRS)對東莨菪堿誘導(dǎo)大鼠干眼模型眼表保護(hù)作用。方法6周齡Wistar大鼠30只(60眼)隨機(jī)分為正常組、干眼組、HRS點(diǎn)眼組、生理鹽水(NS)點(diǎn)眼組、10HRS腹腔注射組和NS腹腔注射組。皮下注射東莨菪堿制備干眼模型。各組行淚液分泌試驗(yàn)(SIt)、淚膜破裂時間(BUT
2、)、角膜上皮熒光素鈉染色評分；行HE染色、過碘酸-雪夫(PAS)染色及免疫組化染色。采用方差分析及LSD檢驗(yàn)。結(jié)果比較各組SIt、BUT及熒光素鈉染色評分差異具統(tǒng)計(jì)學(xué)意義(P<0.05)。HRS點(diǎn)眼組和HRS腹腔注射組均較干眼組改善；且二者差異無統(tǒng)計(jì)學(xué)意義。HE染色示HRS點(diǎn)眼組和HRS腹腔注射組角結(jié)膜上皮水腫減輕；PAS15染色示杯狀細(xì)胞形態(tài)改善；免疫組化染色示鱗狀上皮化生減輕。結(jié)論HRS點(diǎn)眼和HRS腹腔注射均緩解大鼠干眼癥狀，改善角結(jié)膜組織病理損傷，為干眼治療帶來新希望。關(guān)鍵詞：干眼；富氫鹽水；東莨菪堿；大鼠中圖分類號：
3、R7720Theprotectionofhydrogen-richsalineonratdryeyemodelinducedbyscopolaminehydrobromide1122CHUYingying,HUANing,RUYusha,ZHAOShaozhen(1.TianjinMedicalUniversityEyeHospital，SchoolofOptometryandOphthalmologyofTianjinMedicalUniversity，TianjinMedicalUniversityEyeInstitut
4、e，Tianjin300384，China;252.TianjinMedicalUniversityEyeHospital，SchoolofOptometryandOphthalmologyofTianjinMedicalUniversity，TianjinMedicalUniversityEyeInstitute，Tianjin300384)Abstract:ObjectiveToevaluatetheeffectofhydrogen-richsaline(HRS)ondryeyeratinducedbysubcutane
5、ousinjectionofscopolaminehydrobromide.MethodsExperimentresearch.ThirtyfemaleWistarratsaboutsixweeksoldwererandomlydividedintonormalgroup,dryeyegroup,HRS30eyedropsgroup,normalsalineeyedropsgroup(NS),HRSintraperitonealinjectiongroupandNSintraperitonealinjectiongroup,
6、with5ratsineachgroup.Thedryeyeratmodelwasinducedbysubcutaneousinjectionofscopolaminehydrobromideinthelatterfivegroups.Theclinicalsignsofdryeyesuchastearvolume(SIt),tearbreak-uptime(BUT)andcornealepithelialfluoresceinstainingscoreswereevaluatedonday7,14,21and28.Onth
7、e28thday，teneyesineachgroupwereenucleatedand35processedforparaffinsectionsforHEandPASandImmunohistochemistrystaining.ANOVAwasusedtotestthecomparisonsoftheclinicalsigns,andLSDwasusedtotestmultiplecomparisons．ResultsFordryeyegroup,fromthe7thdayuntilthe28thday,theSIt,
8、BUTandfluoresceinstainingscoringofdryeyegroupweresignificantlydifferentfromthenormalcontrol(P<0.05).ForHRSeyedropsgroup,TheBUTwasshorterandfluore
