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1、作物學(xué)報(bào)ACTAAGRONOMICASINICA2016,42(5):684?689http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00684一個(gè)水稻黃綠葉突變基因的定位和遺傳研究12311,*初志戰(zhàn)郭海濱劉小林陳遠(yuǎn)玲劉耀光12華南農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院/亞熱帶農(nóng)業(yè)生物資源保護(hù)與利用重點(diǎn)實(shí)驗(yàn)室,廣東廣州510642;華南農(nóng)業(yè)大學(xué)公共基礎(chǔ)課實(shí)驗(yàn)教3學(xué)中心,廣東廣州510642
2、;宜春學(xué)院,江西宜春33600060摘要:從粳稻品種日本晴經(jīng)Co-γ誘變的M1材料中發(fā)現(xiàn)一個(gè)黃綠葉突變體,其葉片從萌發(fā)到三葉前期表現(xiàn)白化,三葉后期開(kāi)始轉(zhuǎn)為黃綠葉,直到衰老。遺傳分析表明,該突變表型受一對(duì)隱性核基因控制,將該黃綠葉突變體暫定名為ygl8951。與野生型相比,ygl8951的葉綠素含量與類(lèi)胡蘿卜素含量顯著降低。電子顯微鏡觀察表明ygl8951內(nèi)葉綠體數(shù)量明顯減少,葉綠體內(nèi)沒(méi)有基粒類(lèi)囊體,只有類(lèi)似間質(zhì)類(lèi)囊體結(jié)構(gòu)?；虮磉_(dá)定量分析表明,突變體中光系統(tǒng)I和光系統(tǒng)II基因表達(dá)水平明顯下調(diào),核糖體結(jié)構(gòu)基因和質(zhì)體編碼的RNA聚合酶亞基基因表
3、達(dá)明顯上調(diào)。利用ygl8951與秈稻品種黃華占雜交獲得的F2分離群體,將該基因定位于水稻第6染色體上的In/Del標(biāo)記607489與607611之間,物理距離191kb的范圍內(nèi),通過(guò)分析確認(rèn)該基因?yàn)橐粋€(gè)新的調(diào)控葉色的基因。關(guān)鍵詞:水稻;黃綠葉;基因定位;遺傳分析GeneticAnalysisandGeneMappingofaYellow-greenLeafMutantinRice12311,*CHUZhi-Zhan,GUOHai-Bin,LIUXiao-Lin,CHENYuan-Ling,andLIUYao-Guang1CollegeofL
4、ifeSciences,SouthChinaAgriculturalUniversity/StateKeyLaboratoryforConservationandUtilizationofSubtropicalAgro-bioresources,2Guangzhou510642,China;CenterofExperimentalTeachingforCommonBasicCourse,SouthChinaAgriculturalUniversity,Guangzhou510642,3China;YichunUniversity,Yichu
5、n336000,China60Abstract:Ayellow-greenleafricemutant,temporarilynamedasygl8951(yellow-greenleaf8951),wasidentifiedfromCoγ-rayradiationmutationinjaponicaricevarietyNipponbare.Themutantshowedalbinophenotypefromgerminationto3-leaf-stage,thenturnedyellow-greenphenotypetillapopt
6、osis.Thecontentsofchlorophyllandcarotenoidwereobviouslydecreasedinygl8951comparedwiththewildtype.Electronmicroscopeobservationshowedthatnogranathylakoidsbutsomestromathyla-koids-likestructureswerefoundinchloroplastofygl8951mutant.Theexpressionlevelsofsomegenesinvolvedinpho
7、tosystemIandphotosystemIIweredramaticallydecreased,whiletheribosomalandRNApolymerasegenesinchloroplastwereincreasedinygl8951mutantcomparedwiththewildtype.Mapping-basedcloningwasusedtoidentifytheygl8951locususinganF2popula-tionfromacrossingbetweenthemutantandHuanghuazhan.Th
8、eresultshowedthatthemutatedlocuswaslocatedina191kbregiononchromosome6,whichwasassumedtobe
