干旱脅迫下no+對(duì)水稻日本晴葉片生理特性的影響

干旱脅迫下no+對(duì)水稻日本晴葉片生理特性的影響

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時(shí)間:2018-04-15

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1、中國(guó)水稻科學(xué)(ChinJRiceSci),2015,29(1):65-72http://www.ricesci.cnDOI:10.3969/ji.ssn.1001G7216.2015.01.00865干旱脅迫下NO對(duì)水稻日本晴葉片生理特性的影響楊永杰楊雪芹張彩霞符冠富陳婷婷陶龍興?(中國(guó)水稻研究所/水稻生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室,杭州311006;?通訊聯(lián)系人,EGmail:lxtao@fy.hz.zj.cn)EffectsofNitricOxideonDroughtStressGinducedPhysiologicalC

2、haracteristicsinLeavesofNipponbare(OryzasativaL)YANGYongGjie,YANGXueGqin,ZHANGCaiGxia,FUGuanGfu,CHENTingGting,TAOLongGxing?(ChinaNationalRiceResearchInstitute/StateKeyLaboratoryofRiceBiology,Hangzhou310006,China;?Correspondingauthor,EGmail:lxtao@fy.hz.zjc.n)YAN

3、GYongjie,YANGXueqin,ZHANGCaixia,etal.EffectsofnitricoxideondroughtstressGinducedphysiologicalcharacteristicsinleavesofnipponbare(OryzasativaL).ChinJRiceSci,2015,29(1):65G72.Abstract:Torevealthemechanismsthatnitricoxide(NO)enhancesdroughttoleranceinrice,westudie

4、dtheeffectsofNOonleafphotosyntheticrate,relativewatercontentandantioxidantsystemofriceunderdroughtstress.Afterfoliarsprayof100μmol/Lsodiumnitroprusside(SNP,aNOdonor)and/or100μmol/LcPTIO(aNOscavengers),japonicanipponbare(OryzasativaL)wassubjectedtodroughtstressd

5、uringtilleringstageinaplastictank.Riceleavesweresampledforfurtherphysiologicalanalysisatthesixthdayaftertreatment.Resultsshowedthatfoliarsprayof100μmol/LSNPenhancedricetolerancetodroughtstress,whichshowedhigherphotosyntheticrateandrelativewatercontent,butlowerm

6、alonaldehyde(MDA)andhydrogenperoxide(H2O2)contentsinleaveswhencomparedwiththecontrol,foliarsprayingwithdeionizedwater.Bycontrast,thecPTIOtreatmentsignificantlyreducedleafphotosyntheticrateandrelativewatercontent,andsignificantlyincreasedleafMDAandH2O2contents.F

7、urtherstudyindicatedthatfoliarapplicationofSNPsignificantlyincreasedsuperoxidedismutase(SOD)andcatalase(CAT)activities,butobservablysuppressedascorbicacid(AsA)contentandtotalglutathione(GSH+GSSH)contentsunderdroughtstress.However,ithadnosignificantinfluenceonpe

8、roxidase(POD)activity,ascorbateperoxidase(APX)activityandtherewerenosignificantdifferenceamongthefourtreatments.ItwasconcludedthatnitricoxidemightincreasetheactivityofSODand

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