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1、中國水稻科學(ChinJRiceSci),２０１５,２９(４):３３５－３４２http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０４．００１３３５．水稻褐色穎殼新等位基因OsFBX３１０bh６的克隆鑒定徐霞張曉波施勇烽王惠梅黃奇娜奉保華李小紅郭丹吳建利?(中國水稻研究所水稻生物學國家重點實驗室/國家水稻改良中心,杭州３１０００６;?通訊聯系人,EＧmail:beishangd＠１６３．com)bh６,ANewAlleleforBrownHullinRice
2、IdentificationofOsFBX３１０XUXia,ZHANGXiaoＧbo,SHIYongＧfeng,WANGHuiＧmei,HUANGQiＧna,FENGBaoＧhua,LIXiaoＧhong,GUODan,WUJianＧli?(StateKeyLaboratoryofRiceBiology/ChineseNationalCenterforRiceImprovement,ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;?Correspond
3、ingauthor,EＧmail:beishangd＠１６３c．om)bh６,anewalleleforbrownhullinrice．XUXia,ZHANGXiaobo,SHIYongfeng,etal．IdentificationofOsFBX３１０ChinJRiceSci,２０１５,２９(４):３３５Ｇ３４２．Abstract:Abrownhull６(bh６)mutantofricewasisolatedfromanEMSＧinducedIR６４mutantbank．Thebrownpigmentsstart
4、toaccumulateinthehullsafterheadingandreachamaximumlevelinmatureseeds．The１０００Ｇgrainweightandpaniclelengthofbh６significantlydecreasedwhileotheragronomictraitsincludingplantheight,numberofproductivepaniclesperplant,numberoffilledgrainsperpanicleandseedＧsettingrat
5、ewerelargelysimilartothoseofthewildＧtypeIR６４．Analysisofpigmentcontentsindicatedthatthelevelsoftotalflavonoidsandanthocyanininbh６weresignificantlyincreased．Thebrownhullphenotypewascontrolledbyasinglerecessivegeneonthelongarmofchromosome９．Sequencinganalysisindic
6、atedthatasinglebasesubstitution(GＧA)atposition１０１３wasdetectedinthecandidategene(LOC_Os０９g１２１５０)encodinganFＧboxdomaincontainingprotein．Functionalcomplementationusingbh６,isanewalleleofOsFBX３１０thewildＧtypeallelecouldrescuethemutation．Themutation,designatedasOsFBX
7、３１０bh６functioningasaninhibitorofbrownhull．IsolationofOsFBX３１０providesusefulexperimentalmaterialsandwouldfacilitatethestudiesonmechanismofflavonoidmetabolisminmonocotplants．Keywords:OryzasativaL．;brownhullmutant;molecularmarker;OsFBX３１０;flavonoidmetabolism徐霞,張曉
8、波,施勇烽,等．水稻褐色穎殼新等位基因OsFBX３１０bh６的克隆鑒定．中國水稻科學,２０１５,２９(４):３３５Ｇ３４２．摘要:通過EMS誘變獲得遺傳穩(wěn)定的水稻褐色穎殼突變體(brownhull６,簡稱bh６).與野生型IR６４相比,在自然條件下突變體的穎殼顏色自抽穗期開始逐漸加深直至蠟熟期穎殼呈深褐色,除千粒重和穗長顯著降低外,其他農藝性狀(株高、每株有
