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1、中國水稻科學(xué)(ChinJRiceSci),２０１６,３０(３):３０４－３１２http://www．ricesci．cn３０４DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１５０粳稻超親變異系籽粒谷氨酰胺合成酶基因表達(dá)特性及序列變異分析徐振華１,２曲瑩１劉海英１,２朱立楠１張忠臣１金正勛１,*(１東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)院,哈爾濱１５００３０;２黑龍江省農(nóng)業(yè)科學(xué)院五常水稻研究所,黑龍江五常１５０２２９;?通訊聯(lián)系人,EＧmail:zxjin３２６＠hotmail．com)ExpressionCharacteristicsandSequenceVariationAnalysisofG
2、lutamineSynthetaseGeneinGrainofjaponicaRicewithTransgressiveVariationXUZhenＧhua１,２,QUYing１,LIUHaiＧying１,２,ZHULiＧnan１,ZHANGZhongＧchen１,JINZhengＧxun１,*(１NortheastAgriculturalUniversity,Harbin１５００３０,China;２WuchangRiceResearchInstitute,HeilongjiangAcademyofAgriculturalSciences,Wuchang１５０２２９,China;?Cor
3、respondingauthor,EＧmail:zxjin３２６＠hotmailc．om)XUZhenhua,QUYing,LIUHaiying,etal．Expressioncharacteristicsandsequencevariationanalysisofglutaminesynthetasegeneingrainofjaponicaricewithtransgressivevariation．ChinJRiceSci,２０１６,３０(３):３０４Ｇ３１２．Abstract:Thetransgressivevariantsderivedfromricevarietieswiths
4、ignificantdifferenceingrainproteincontentwereusedtoinvestigatetheexpressionlevelandstructureofglutaminesynthetasegene(GS),activityofglutaminesynthetaseandproteinaccumulationcharacteristicsduringgrainfilling．Theresultsshowedthattransgressivevariantscouldbeobtainedthroughsuccessivedirectiveselection
5、ofgrainproteincontent,becausethegrainproteincontentwascloselyrelatedtocorrespondinggenotype．Duringgrainfilling,theactivityofglutaminesynthetasefollowedasingleＧpeakcurve,highlycorrelatedwithgrainproteincontentandsignificantlydifferedbetweenparentsandprogenies．GS１．３andGS２genes,intransgressivevariant
6、sandtheirparentswithdifferentproteincontent,exhibitedsimilartranscriptiontrendduringgrainfilling,thatis,thetranscriptionlevelincreased１５－２０daysbeforeheadingandthendecreasedgradually．Moreover,thegrainproteincontentwascloselyrelatedtoGS１．３andGS２expressionlevels．ThevarietieswithhigherGStranscriptionl
7、evelshowedhigherproteincontentcomparedwiththeirparents．Inaddition,althoughGS１．３andGS２genesequencesshowedhighconservation,thegenesequenceandproteinsequenceofGS１．３andGS２werenotcompletelyidenticalindifferentvarietie
