資源描述：
《力達(dá)霉素對(duì)人宮頸癌caski細(xì)胞增殖的影響及其機(jī)制》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、力達(dá)霉素對(duì)人宮頸癌Caski細(xì)胞增殖的影響及其機(jī)制嘩麵占景瓊?cè)龒{大學(xué)腫瘤微環(huán)境與免疫治療湖北省重點(diǎn)實(shí)整室R的探討力達(dá)霉素對(duì)人宮頸癌Caski細(xì)胞增殖的影響及其可能的機(jī)制。方法將對(duì)數(shù)生長(zhǎng)期的人宮頸癌Caski細(xì)胞隨機(jī)分為低、中、高濃度組及對(duì)照組，低、中、高濃度組均加入含力達(dá)霉素的RPM11640完全培養(yǎng)基100PL，分別調(diào)整其終濃度為1、2.5、5ng/mL,對(duì)照組僅加入等體積RPMI1640完全培養(yǎng)基。作用24h,采用MTT法檢測(cè)細(xì)胞增殖能力（以吸光度值表示），計(jì)算細(xì)胞生存率;采用流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期及線粒體膜電位降低比例;透射電子顯微鏡不觀察細(xì)胞形態(tài)學(xué)改變，計(jì)算細(xì)胞凋亡率及裂亡率
2、。結(jié)果低、中、高濃度組細(xì)胞增殖能力及細(xì)胞生存率均低于對(duì)照組，且高濃度組降低更明顯（P均＜0.01）。對(duì)照組及中濃度組S期細(xì)胞比例均高于低、高濃度組（P均〈0.05）。低、中、高濃度組G0/G1期細(xì)胞比例均低于對(duì)照組，G2/M期細(xì)胞比例均高于對(duì)照組，且中、高濃度組變化更明顯（P均〈0.05）。低、屮、高濃度組及對(duì)照組細(xì)胞線粒體膜電位降低比例分別為（34.77±5.33）%、（37.87±5.73）%、（18.37±3.91）%、（4.17±0.65）%;低、中、高濃度組細(xì)胞線粒體膜電位降低比例均高于對(duì)照組，且低、中濃度組均高于高濃度組（P均＜0.05）。透射電鏡下可見對(duì)照組細(xì)胞形態(tài)正常
3、，低、中、高濃度組存在多種形態(tài)的死亡細(xì)胞，包括凋亡、壞死、裂亡的細(xì)胞。對(duì)照組及高、低、屮濃度組細(xì)胞凋亡率及裂亡率均依次升高，兩組間比較P均〈0.05。結(jié)論力達(dá)霉素可抑制人宮頸癌Caski細(xì)胞增殖，并呈濃度依賴性，其機(jī)制可能與誘導(dǎo)細(xì)胞凋亡或裂亡有關(guān)。關(guān)鍵詞：宮頸癌;力達(dá)霉素;人宮頸癌Caski細(xì)胞;細(xì)胞凋亡;細(xì)胞裂亡;線粒體膜電位；E-mai1:4554080@qq.基金：國(guó)家自然科學(xué)基金資助項(xiàng)目(81372265)EffectoflidamycinonproliferationofhumancervicalcancerCaskicellsLUHuaQINYeZHANJingqiong
4、WANGYanlinChinaThreeGorgesUniversity,HubeiKeyLaboratoryofTumorMicroenvironmentandImmunotherapy;Abstract：ObjectiveToinvestigatetheeffectoflidamycinontheproliferationofhumancervicalcancerCaskicellsanditsunderlyingmolecularmechanisms.MethodsHumancervicalcancerCaskicellswererandomlydividedtheexperi
5、mentalgroups(includingthelow-dose,medium-doseandhigh-dosegroups)whichwereseparatelytreatedwith1,2.5and5ng/mLlidamycin(100uL)for24h,andthecontrolgroupwhichwasaddedwiththesamevolumeofRPMI-1640.MTTassaywasusedtodetecttheinhibitionrateofproliferation.ThecellcycleandmitochondrialmembranepotentialofC
6、asKicellsweremeasuredbyflowcytometry.TransmissionelectronicmicroscopewasusedtoobservethemorphologicalchangesofCaskicells.Wecalculatedtheapoptosisrateandmitoticcatastropherate.ResultsTheproliferationrateandsurvivalrateoftheexperimentalgroupsweresignificantlylowerthanthoseofthecontrolgroup,andthe
7、decreaseinthehigh-dosegroupwasmoresignificant(allP<0.05).ThepercentageofcellsintheSphaseofthecontrolgroupandthemedium-dosegroupwashigherthanthoseinthelow-doseandhigh-dosegroups(allP<0.05).ThepercentageofcellsintheG0/G1phaseoftheex
