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1、淫羊藿提取物的雌激素樣作用研究第30卷第3期2009年6月西安交通大學(xué)(醫(yī)學(xué)版)JournalofXi'anJiaotongUniversity(MedicalSciences)Vo1.30No,3Jun.2009淫羊藿提取物的雌激素樣作用研究于燕,顏虹,胡森科,張敬華(西安交通大學(xué)醫(yī)學(xué)院公共衛(wèi)生系,陜西西安710061)摘要:目的評(píng)價(jià)淫羊藿提取物的雌激素樣作用.方法采用小鼠子宮增重實(shí)驗(yàn),雌激素受體陽(yáng)性細(xì)胞系(MCF一7)細(xì)胞增殖及拮抗實(shí)驗(yàn),評(píng)價(jià)淫羊藿及淫羊藿苷的雌激素樣作用及其可能機(jī)制.結(jié)果與陰性對(duì)照組比較,淫羊藿及淫羊藿
2、苷能使子宮系數(shù)由0.097±0.008明顯增加至0.156±0.018及0.152±0.016,且用藥前,后動(dòng)物體重由(18.9±1.3)g分別增至(19.8±1.3)g及(2O.03±1.3)g,增幅明顯高于陰性列照組(P<0.05);淫羊藿苷可使血清雌激素E山(132.22±11.45)ng/L增高至(147.73±12.43)ng/I,FSH水平由(50.15±3.36)t~g/L降低至(44.47±2.46)g/!(P<O.05),但淫羊藿組雌激素E.水平無(wú)明顯改變;在24,48,72h,淫羊藿及淫羊藿苷
3、對(duì)MCF一7細(xì)胞的增殖均打叫顯的促進(jìn)作用(P<0.05),分別由0.238±0.043,0.387±0.051,0.563±0.083增至0.362±0.045,0.521±O.046,0.648±0.043及0.324±0.043,0.465±0.034,0.622±0.079;72h加入ICI后,細(xì)胞增殖明顯受到抑制,細(xì)胞增『『【}-半分別降至99.461,94.434.結(jié)論淫羊藿及淫羊藿營(yíng)具有雌激素樣作用,且其促細(xì)胞增殖作用可能是通過(guò)雌激素受休(ER)介導(dǎo)產(chǎn)生的.關(guān)鍵詞:淫羊藿;提取物;雌激素樣作用中圖分類(lèi)號(hào):R
4、284;R285.5文獻(xiàn)標(biāo)志碼:A文章編號(hào):1671—8259(2009)03037304Studyontheestrogen—likeeffectsofEpimediumextractiveYANHong,HUSenke,ZHANGJing—huaMedicalSchoolofXi'anJiaotongUniversity,Xi'an710061.China)ABSTRACT:ObjectiveToinvestigatetheestrogen.1ikeeffectsofEpimediumextractive.Methods
5、ByusingtheuterusgrowthtestinmiceandtestofMCF一7proliferationandaddingestrogenreceptorantagonistIcariin,weevaluatedtheestrogeniceffectsofEpimediumandIcariinandthepossiblemechanism.ResultsEpimediumandIcariinincreasedsignificantlytheuteruscoefficientinmicefrom0.097±0.0
6、08to0.156±0.018and0.152±0.016,respectively:theweightincreasedsignificantlyfrom(18.9±1.3)gto(19.8±1.3)gand(20.03±1.3)g(P<O.05).IcariinincreasedsignificantlytheserumlevelofE2from(132.22±11.45)ng/Lto(147.73±】2.43)ng/LanddecreasedFSHlevelfrom(50.15±3.36)g/Lto(44.47±
7、2.46)g/L(P<o.05),buttherewasnosignificantdifferenceinEpimediumgroup.ThercwasobviouslyenhancedproliferationinMCF一7in24,48and72h(P<0.05),from0.238±0.043,0.387±0.051and0.563±0.083to0.362±0.045,0.521±0.046and0.6484-_0.043aswellas0.324土0.043,0.465±0.034and0.622±0.
8、079,respectively.However,thecellproliferationwasinhibitedsignificantlybyIcariinaddedin72h-theproliferationratiodecreasedto99.461%and94.434%?respe
