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《三種骨移植材料的骨誘導(dǎo)活性對(duì)比實(shí)驗(yàn)研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、三種骨移植材料的骨誘導(dǎo)活性對(duì)比實(shí)驗(yàn)研究作者：游永剛 徐永清 唐輝，趙萬秋，陸華拓，張勇，李剛，趙璽龍，陳蘇麗【摘要】 目的通過對(duì)人工骨硫酸鈣(CS)、同種異體和異種脫礦骨(DBM)3種骨移植材料異位誘導(dǎo)成骨效應(yīng)的觀察，比較其骨誘導(dǎo)活性優(yōu)劣，為臨床選擇合適的骨移植材料提供參考依據(jù)。[方法]采用大鼠股部肌袋內(nèi)埋植實(shí)驗(yàn)。選擇成年斯普拉大鼠共36只，隨機(jī)分成A、B2組，每組18只。A組左側(cè)植入CS(A1)，右側(cè)植入同種異體DBM(A2);B組左側(cè)植入異種DBM(B1)，右側(cè)不植入任何材料行空白對(duì)照(B2)。通過異位誘導(dǎo)成骨實(shí)驗(yàn)，在植入后2、4、
2、6周取材作大體、組織形態(tài)學(xué)觀察及堿性磷酸酶(ALP)和鈣離子生化測(cè)定，對(duì)不同材料的骨誘導(dǎo)活性進(jìn)行評(píng)價(jià)。[結(jié)果]術(shù)后2周，2種DBM被纖維組織包裹，可觀察到DBM骨片周圍間充質(zhì)細(xì)胞聚集;4周時(shí)可見軟骨細(xì)胞、成骨細(xì)胞形成;6周時(shí)有類似軟骨基質(zhì)樣物質(zhì)形成;ALP和鈣離子含量在各時(shí)間段均高于對(duì)照組，說明2種DBM具有骨誘導(dǎo)活性，其結(jié)果差異具有供體依賴性。而CS降解吸收快，炎癥反應(yīng)輕，未見誘導(dǎo)成骨現(xiàn)象。[結(jié)論]2種DBM具有骨誘導(dǎo)活性，同種異體DBM較好，異種DBM次之，供體的差異性和DBM的制備可影響其在體內(nèi)的骨誘導(dǎo)活性。CS生物相容性較好，是可
3、供選擇的骨修復(fù)填充材料?！娟P(guān)鍵詞】脫礦骨硫酸鈣骨移植骨誘導(dǎo)活性14 Abstract：[Objective]Tocomparetheosteoinductiveactivityofcalciumsulfate(CS),allogeneticdemineralizedbonematerials(ADBM)andheterogeneticdemineralizedbonematerials(HDBM)byobservingtheirefficiencyininducingboneformation.[Method]CS,ADBMandHDB
4、Mweretransplantedintothighmusclepouchesofmice.ThirtysixmatureSpragueDawlymiceweredividedinto2groupsatrandom.CSwastransplantedintotheleft(groupA1,n=9)andADBMintotheright(groupA2,n=9)thighmusclepouches.HDBMwastransplantedintotheleftthighmusclepouches(groupB1,n=9)andtherig
5、htsitesweretakenasblankcontrols(groupB2,n=9).Experimentsweredonetoinduceectopicboneformation.At2,4,6weekspostoperatively,specimenwerecollectedtoevaluategrossandtissuestructuresandbiochemicaltestsforalkalinephosphatase(ALP)andCa2+sothatosteoinductiveactivitiesofdifferentbo
6、negraftmaterialscouldbeassessed.[Result]At2weekspostoperativly,ADBMandHDBMwerewrappedupbyfibroustissuesandstromalcellsgatheringaroundtheDBMslices.At4weekspostoperativly,formationofcartilageandosteoblastswereobserved,andat6weeks,materialslikecartilagematrixwereobservedtogr
7、ow.TheconcentrationofALPandCa2+instudy14groupswashigherthanthatofcontrolgroup,whichmeantthat2typesofDBMhadosteogenicpotentialandthatthedifferencesofosteogenetiepotentialinADBMandHDBMreliedonthedonors,whereasCScouldbedegradedandabsorbedfastwithlightinflammatoryreactionandn
8、oectopicboneformationwasobservedinCSgraft.[Conclusion]BothADBMandHDBMhaveosteoinductivepotential
