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《基于石墨烯納米復(fù)合材料的生物傳感器分析》由會員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、英文摘要current—time(i-t)curvemethod.WeinvestigatedtheeffectofPBandRGOonthesensitivityofsensor,anddiscussedanti.interferenceandclinicalapplicationofthesensor.PartII:Constructionofpesticidebiosensorbasedonprussianblue/graphene—goldnanoparticals-p—cyclodextrin(PB/RGO-AuNPs。p。CD)compositemembran
2、e.Step1:ElectrochemicaldepositionofRGO-AuNPs-p—CDmembraneontheelectrodewerecarriedoutbycontrolledpotentialmethod.Step2:ElectrochemicaldepositionofPB.CSmembraneonthemodifiedelectrodewascarriedoutbyCV.Step3:TheCSwithgoodbiologicalcompatibilitywasusedasacarrierforthehi曲lyefficientimmobilizat
3、ionofAChE.OrganophosphoruspesticideandcarbamatepesticidesareusuallydetectedbasedonmeinhibitionoftheAChEbythesecompounds.TheinhibitionoforganophosphoruspesticideandcarbamatepesticidesonAChEresultsindecreaseofproducedthiocholine,andtheoxidationcurrentdecreasesaccordingly.Theorganophosphorus
4、pesticideandcarbamatepesticidesCanbedetectedbymeasuringthedeclineoftheoxidationcurrentofthiocholine.Theelectrodesurfacewascharacterizedbyscanningelectronmicroscopy(SEM)method.Thecurrentresponsesofthebiosensortoacetylthiocholinechloridewereresearchedbythei-tcurvemethod.Theeffectofinhibitio
5、ntime,AChEreactivation,stability,selectivityandrepeatabilityofsensorwerediscussed,andfinallythebiosensorwasusedtodetecttherecoveryoftherealsamples.Results:1Preparation,characterizationofgrapheneanditsapplicationinglucosebiosensors1.1Graphenepreparedbythisexperimentwasstableandcouldbedirec
6、tlyusedinthemodificationoftheelectrodewithoutanytreatment.AsshowninXRD,thecrystalstructureofgraphitewasdestroyed,whilecrystalintegritydeclinedandtheflakelayerbecamesmallerduringtheredoxprocess,英文摘要.indicatingthegraphenewasobtained.Ultravioletabsorbancespectrashowedthattheoxygen.containing
7、functionalgroupsonthesurfaceofthegrapheneoxidewasreducdedgraduallyandⅡ-JIconjugatesystemwasrestored,whichdemonstratedthesuccessfulpreparationofgrapheneonceagain.TheelectroconductivityofdifferentmodifiedelectrodessurfacewascharacterizedbvCV.Theresultsshowthattherespo