冠狀病毒Coronavirusl論文-1987 Characteristics of Australian human enteric coronavirus-like particles_ comparison with human respiratory coronavir.pdf

冠狀病毒Coronavirusl論文-1987 Characteristics of Australian human enteric coronavirus-like particles_ comparison with human respiratory coronavir.pdf

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1、ArchVirol(1987)97:309-323ArchivesofVirology?bySpringer-Verlag1987CharacteristicsofAustralianhumanentericcoronavirus-likeparticles:comparisonwithhumanrespiratorycoronavirus229EandduodenalbrushbordervesiclesR.D.Schnagl1,SharonBrookes1,SueMedvedec1,andFranMorey2IDepartmentofMicr

2、obiology,LaTrobeUniversity,Bundoor~,Victoria,and2PathologyLaboratory,AliceSpringsHospital,AliceSprings,NorthernTerritory,AustraliaAcceptedJuly27,1987SummaryThepolypeptideprofilesofhighlypurifiedcorona.virus-likeparticles(CVLPs)provedtobeverydifferentfromthatofhumanrespiratory

3、coronavirus229Eandshowedtheparticlesnottobecoronaviruses.Dif-ferencesinpolypeptideprofilesandmorphologybetweentheCVLPsandduodenalbrushbordervesiclessuggestedthattheCVLPswerealsonotsuchvesicles.Althoughtheysharedsomebasicoverallsimilarity,thepolypeptideprofilesofthreedifferent

4、butpossiblyantigenicallyidenticalCVLPprep-arationsfromCentralAustralianAborigineswereverydissimilarindetail.Atleast38,39and48bandsrespectivelywereobservedonthethreeprofiles.Atleast46bandswerevisibleonthepolypeptideprofileofCVLPsfromaVietnameseimmigranttoAustralia,anditalsodif

5、feredindetailfromthoseoftheCentralAustralianCVLPs.IndicationsofantigenicdifferencewereobtainedbetweenCentralAus-tralianCVLPsandCVLPsfromIndia,Kiribati,SouthAfricaandViet-nameseimmigrantstoAustralia.AntigenicdifferencewasalsosuggestedbetweentheCentralAustralianCVLPsandthoset~'

6、omonedistantlo-cationwithinAustralia,butantigenicsimilaritywiththosefromanotherwasindicated.IntroductionSincethedetectioninhumanstoolsofpleomorphicparticlesresem-blingcoronavirusesin1975[6,15]manyfurtherobservationsofsuchparticlesinf~eceshavebeenmade.Morerecentlyithasbecomecl

7、earer310g.D.Schnagletal.thatsomeoftheseparticlesaremostprobablytrueeoronaviruses[9,16],whileothersarelikelytofitintotherecentlyproposedtorovirusgroup[3,23].Manyotheroftheseobservedhumanentericeoronavirus~likeparticles(CVLPs),oftypeswhichappearedtobemorphologicallymoredissimil

8、artotheclassicalcoronaviruseshave,however,notyetbeenabletobeadequate

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