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1、白個菌耐干旱砲了液體培養(yǎng)條件研究白僵菌耐干旱砲了的液體培養(yǎng)條件研究Drought-resistantsporesofBeauveriabassianaLiquidCultureConditions摘要白僵菌是一類以昆蟲為寄主的具有好氣性的病原真菌。片僵菌在真菌分類學(xué)上屬于半知菌，絲他綱、絲他目、絲他科，白僵菌屬，通常根據(jù)雀了的形態(tài)分為球砲白僵[Beauveriabassiana(Bais.)Vuill?]和卵抱白僵菌Beauveriatenell(Del.)Siem.,其屮球泡白僵菌作為真菌殺蟲劑已經(jīng)被廣泛研究和應(yīng)用于生
2、物防治農(nóng)林果蔬等多種作物害蟲，是真菌殺蟲劑應(yīng)用最多的一個種類。白個菌在持續(xù)長久控制害蟲，保存多種生物種類，維護(hù)生態(tài)平衡方面具有不可替代的作用，它對人畜無毒、無害、無副作用，不致畸、不致癌、不污染環(huán)境，是一類比較理想的綠色環(huán)保型生物殺蟲劑。80對白僵菌液生分生抱了的形成具有顯著的影響，培養(yǎng)基屮吐溫80含量在0.4%?0.8%之間時，有利于液生分生淪了的形成，產(chǎn)淪量(n)達(dá)7.30x1010?24.13"010L-1。關(guān)鍵詞：白僵菌；分生砲子；液體培養(yǎng)AbstractBeauveriabassianaisakindofins
3、ecthostwithagoodairofpathogenicfungi.FungusBeauveriabassianainthetaxonomyofimperfectfiingibelongingtosub-door,Trichosporonclasses,Trichosporonhead,TrichosporonBranch,BeauveriabassianaisusuallydividedaccordingtotheformofsporesofBeauveriawhitecadaver[Beauveriabassi
4、ana(Bais.)Vuill.]andeggbassianaBeauveriatendI(Del.)Siem.,asthefungusBeauveriabassianawhichpesticideshavebeenwidelystudiedandusedinavarietyoffruitsandvegetablessuchasagriculture,forestrybiologicalcontrolofcroppests,istokillfungusoneofthelargestpesticideapplication
5、category.Beauveriabassianainthecontinuinglong-termcontrolofpests,saveavarietyofspeciesandmaintainingecologicalbalancehasanirreplaceablerole,itshumanandanimalnon-toxic,harmlessandwithoutsideeffects,donotteratogenic,notcarcinogenic,donotpollutetheenvironment,isakin
6、dofidealgreenenvironmentalprotectionofbiologicalpesticides.Inthispaper,astrainofBeauveriabassianaSZ21experimentalmaterials,thepreparationofdiflerenttypesandconcentrationsofcarbon,nitrogen,inorganicsaltsliquidmediumcombination,dubbedthe4x106spores/mLofthesporesusp
7、ension;takeafew250mLflaskfittedtakeondifferentliquidmedia,respectively,each75mL,ImLsporesuspensioninoculatedbottle;300rpm,25±1°Cundertheconditionsofshaking,eachtreatmentrepeatedthreetimesdailytodetectandrecordtheliquidmediumfromspores?Theresultsshowedthatcarbonan
8、dnitrogensourcesontheformationofBcauvcriabassianaconidiaLiquidstudentshaveasignificantimpact.Glucose,sucrose,lactoseisagoodliquidculturesporecarbon,andsoybeanm
