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1、反義核酸抑制RON基因表達(dá)對(duì)A549的體外作用第l4卷第24期2004年12月中國(guó)現(xiàn)代醫(yī)學(xué)雜志ChinaJournalofModemMedicineVo1.14No.24Dec.2oal4文章編號(hào):1005-8982(2004)24—0001—05InVitroinhibitionofanti---sensenucleicacidofoncogeneRONonlungcancercelllineA549PANTie-cheng,XULi-jun,LIJun,ZHAOJin—ping,CHENTao,ZHANGNi,WEIXiang,PA
2、NYou—min,LIULi—gang(DepartmentofCardiothroracicSurgery,TondiHospital,Ton~iMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan,Hubei430030,P.R.China)?論著?Abstract:Objective:ToinvestigatetheeffectofInVitroinhibitionofRONgeneexpressionandcellgrowtheffectoflungcance
3、rcellhneA549,anti-sensenucleicacidoftransmembranedomainofRONgene(RONm)wasintroducedintothiscellline.Thepossibilityoftheanti-sensonucleicacidofgeneR0NasacandidateforlungcancergenetherapyWasalsoexplored.Methods:RONmcDNAWasinverselyinsertedineukaryoficexpressionvectorpcDNA3
4、.1.thantheanti—sensenucleicacidofthisdomainWastransferredintothestudiedceils.TheexpressionofRONgeneinthisexperimentandcontrolgroupsweremonitoredbyEUSAandthealterationofcellproliferationWascheckedbyM,ITrassayetc.Results:TheexpressionlevelofRONgeneinthetransfectedlungcance
5、rcellsWasreducedandthecellproliferationWasinhibitedcomparingwiththecontrolcelllines.Conclusion:Theanti—sensenucleicacidofRONmsignificantlyreducestheexpressignofRONgeneinthehighlyexpressedlungcancercellfineA549andmightinhibitthegrowthofthiscellline.Keywords:GeneRON;anti——
6、sensenucleicacid;anti.—sense;lungcancer,restrainCLCnumber:R733.7Documentcode:A反義核酸抑制RON基因表達(dá)對(duì)A549的體外作用潘鐵成,徐利軍,李軍,趙金平,陳濤,張霓,魏翔,潘友民,劉立剛(華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院胸心外科,湖北武漢430030)摘要:目的探討針對(duì)人RoN基因跨膜區(qū)段(RONm)反義核酸對(duì)肺癌細(xì)胞系A(chǔ)549活性和細(xì)胞增殖的抑制作用,研究以RONm為靶的肺癌基因治療的可能性?方法將人RONmcDNA反向插入到真核表達(dá)栽體pcDNA3.1中
7、,轉(zhuǎn)染肺癌細(xì)胞A549,利用ELISA檢測(cè)細(xì)胞模型RON基因表達(dá)水平,同時(shí)利用MTT和細(xì)胞計(jì)數(shù)方法監(jiān)測(cè)細(xì)胞生物學(xué)活性的變化.結(jié)果轉(zhuǎn)染后的肺癌細(xì)胞RON基因表達(dá)量和細(xì)胞活性明顯降低,并出現(xiàn)明顯的細(xì)胞凋亡?結(jié)論RONm反義核酸明顯抑制RON基因的表達(dá),同時(shí)對(duì)肺癌細(xì)胞的生長(zhǎng)有抑制作用,促進(jìn)細(xì)胞凋亡?關(guān)鍵詞:R0N基因;反義核酸;反義;肺癌;抑制中圖分類號(hào):R733.7文獻(xiàn)標(biāo)識(shí)碼:ARON(RecepteurdOrigineNantais)gene,aMETproto—oncogenefamilymember,encodingareceptor
8、tyrosinekinaseRON—RTK,hasbeenimplicatedintheprogressionofcertainepithelialcancers.RON—RTKwasfoundwidely
