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1、組織工程骨修復山羊負重骨大段骨缺損的長期觀察作者：陳濱1；裴國獻1；王珂2；唐光輝1 (1南方醫(yī)科大學南方醫(yī)院創(chuàng)傷骨科，廣東廣州510515；2解放軍153中心醫(yī)院骨科，河南鄭州450042)摘要：目的??探討組織工程骨修復山羊大段負重骨骨缺損的長期效果及所用支架材料珊瑚羥基磷灰石的體內最終轉歸情況。方法??中國青山羊15只，制備單側脛骨2cm的骨膜與骨缺損，缺損內植入組織工程骨(珊瑚羥基磷灰石+經(jīng)誘導分化的骨髓基質干細胞)。術后早期行ECT、X線、組織學等手段檢測，評價骨缺損修復情況。遠期在術后6、12、1
2、8、24月行X線及組織學檢查，評價骨缺損修復情況及珊瑚羥基磷灰石的體內轉歸。結果??早期ECT顯示在術后2個月內骨再生和再血管化進展順利，X線和組織學顯示術后組織工程骨成骨呈漸進性和偏心性；遠期X線和組織學顯示組織工程骨與山羊脛骨牢固愈合，并開始塑形且出現(xiàn)髓腔再通，珊瑚羥基磷灰石在體內逐漸成為骨基質的組成成分，自身架構消失。結論??組織工程骨可以完全修復山羊大段負重骨骨缺損，形成正常骨組織并發(fā)揮功能；珊瑚羥基磷灰石最終被降解轉化成骨基質。關鍵詞：骨組織工程；山羊；脛骨/損傷；骨缺損；珊瑚羥基磷灰石；骨髓基質干細
3、胞中圖分類號：R687.3;R318.17??文獻標識碼：A??文章編號：1673-4254(2006)06-0770-04 Long-termobservationoflargeweight-bearingbonedefectingoatsrepairedwithtissueengineeringtechniqueCHENBin1；PEIGuo-xian1；WANGKe2；TANGGuang-hui1；1DepartmentofOrthopedicsandTraumatology,NanfangHospita
4、l,SouthernMedicalUniversity,Guangzhou510515,China;2DepartmentofOrthopedics,153HospitalofPLA,Zhengzhou450042,ChinaAbstract:Objective??Toobservethelong-termeffectoftissueengineering-basedrepairoflargeweight-bearingbonedefectingoats,andthefinaloutcomeofthescaff
5、oldmaterialcoralhydroxyapatite(CHAP)??invivo.Methods??FifteenChinesegoatsweresubjectedtooperationstoinducea2-cmlefttibialdiaphysealdefect,whichwasfilledsubsequentlywithCHAPandbonemarrowstromalstemcells(BMSCs).TherepaireddefectswereevaluatedbyECT,X-rayandhist
6、ologyintheearlystageandat6,12,18,and24monthspostoperatively.Results??ECTshowedgoodboneregenerationandrevascularizationwithin2monthspostoperatively.X-rayandhistologydisplayedeccentricandgradualboneregenerationintheearlystage,andthetissue-engineeredbonegraftwa
7、sfirmlyhealedwiththegoattibia.X-rayandhistologicalexaminationat6,12,18,24monthspostoperativelyrevealedmouldingofthenewbonesandmedullarycavityrecanalization,andthestructureofCHAPdisappearedandgraduallyintegratedintothenewbones.Conclusions??Tissue-engineeredbo
8、neiscapableoftotalrepairoflargebonedefectingoatsbyformingnormalfunctionalnewbones.CHAPcanbeeventuallydegradedcompletelyandbecomethecomponentofthenewlygeneratedbones.Keywords:tissueengineeringbon
