資源描述：
《組織工程骨修復(fù)山羊負(fù)重骨大段骨缺損的長期觀察》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、組織工程骨修復(fù)山羊負(fù)重骨大段骨缺損的長期觀察作者：陳濱1；裴國獻(xiàn)1；王珂2；唐光輝1 (1南方醫(yī)科大學(xué)南方醫(yī)院創(chuàng)傷骨科，廣東廣州510515；2解放軍153中心醫(yī)院骨科，河南鄭州450042)摘要：目的??探討組織工程骨修復(fù)山羊大段負(fù)重骨骨缺損的長期效果及所用支架材料珊瑚羥基磷灰石的體內(nèi)最終轉(zhuǎn)歸情況。方法??中國青山羊15只，制備單側(cè)脛骨2cm的骨膜與骨缺損，缺損內(nèi)植入組織工程骨(珊瑚羥基磷灰石+經(jīng)誘導(dǎo)分化的骨髓基質(zhì)干細(xì)胞)。術(shù)后早期行ECT、X線、組織學(xué)等手段檢測，評價骨缺損修復(fù)情況。遠(yuǎn)期在術(shù)后6、12、18、24月行X線及組織學(xué)檢查，評價骨缺損修復(fù)情況及珊瑚羥基磷灰石的體內(nèi)轉(zhuǎn)歸。結(jié)果
2、??早期ECT顯示在術(shù)后2個月內(nèi)骨再生和再血管化進(jìn)展順利，X線和組織學(xué)顯示術(shù)后組織工程骨成骨呈漸進(jìn)性和偏心性；遠(yuǎn)期X線和組織學(xué)顯示組織工程骨與山羊脛骨牢固愈合，并開始塑形且出現(xiàn)髓腔再通，珊瑚羥基磷灰石在體內(nèi)逐漸成為骨基質(zhì)的組成成分，自身架構(gòu)消失。結(jié)論??組織工程骨可以完全修復(fù)山羊大段負(fù)重骨骨缺損，形成正常骨組織并發(fā)揮功能；珊瑚羥基磷灰石最終被降解轉(zhuǎn)化成骨基質(zhì)。關(guān)鍵詞：骨組織工程；山羊；脛骨/損傷；骨缺損；珊瑚羥基磷灰石；骨髓基質(zhì)干細(xì)胞中圖分類號：R687.3;R318.17??文獻(xiàn)標(biāo)識碼：A??文章編號：1673-4254(2006)06-0770-04 Long-termobservat
3、ionoflargeweight-bearingbonedefectingoatsrepairedwithtissueengineeringtechniqueCHENBin1；PEIGuo-xian1；WANGKe2；TANGGuang-hui1；1DepartmentofOrthopedicsandTraumatology,NanfangHospital,SouthernMedicalUniversity,Guangzhou510515,China;2DepartmentofOrthopedics,153HospitalofPLA,Zhengzhou450042,ChinaAbstrac
4、t:Objective??Toobservethelong-termeffectoftissueengineering-basedrepairoflargeweight-bearingbonedefectingoats,andthefinaloutcomeofthescaffoldmaterialcoralhydroxyapatite(CHAP)??invivo.Methods??FifteenChinesegoatsweresubjectedtooperationstoinducea2-cmlefttibialdiaphysealdefect,whichwasfilledsubseque
5、ntlywithCHAPandbonemarrowstromalstemcells(BMSCs).TherepaireddefectswereevaluatedbyECT,X-rayandhistologyintheearlystageandat6,12,18,and24monthspostoperatively.Results??ECTshowedgoodboneregenerationandrevascularizationwithin2monthspostoperatively.X-rayandhistologydisplayedeccentricandgradualbonerege
6、nerationintheearlystage,andthetissue-engineeredbonegraftwasfirmlyhealedwiththegoattibia.X-rayandhistologicalexaminationat6,12,18,24monthspostoperativelyrevealedmouldingofthenewbonesandmedullarycavityrecanalization,andthestructureofCHAPdisappearedandgraduallyintegratedintothenewbones.Conclusions??T
7、issue-engineeredboneiscapableoftotalrepairoflargebonedefectingoatsbyformingnormalfunctionalnewbones.CHAPcanbeeventuallydegradedcompletelyandbecomethecomponentofthenewlygeneratedbones.Keywords:tissueengineeringbon
