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1、磷酸鹽緩沖液（PBS）配制方法0.01MPBS?PBS(135mMNaCl,2.7mMKCl,1.5mMKH2PO4,and8mMK2HPO4，pH7.2)PBS緩沖液（pH7.2～7.4）：NaCl137mmol/L，KCl2.7mmol/L，Na2HPO44.3mmol/L，KH2PO41.4mmol/L稱7.9gNaCl，0.2gKCl，0.24gKH2PO4(or1.44gNa2HPO4)和1.8gK2HPO4，溶于800ml?蒸餾水中，用HCl調(diào)節(jié)溶液的pH值至7.4，最后加蒸餾水定容至1L。保存于4℃冰箱中即可。需要注意的是，通常所說的濃度0.01M指的是緩沖溶液中所有的磷酸根濃度
2、，而非Na離子或K離子的濃度，Na離子和K離子只是用來調(diào)節(jié)滲透壓的。母液的配制：?0.2MNa2HPO4：稱取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：稱取31.2gNaH2PO4-2H2O，溶于1000ml水各種濃度PB(pH=7.4)的配制：先配0.2MPB(pH=7.4，100ml)：取19ml0.2mol/L的NaH2PO4，?81ml0.2mol/L的Na2HPO4,即可。然后只需將0.2MPB(pH=7.4)按相應(yīng)比例適當(dāng)稀釋即可，如：0.1MPB（PH=7.4）：取500ml0.2MPB，加水稀釋至1000ml即可。0.01MPB（PH=7.
3、4）：取50ml0.2MPB，加水稀釋至1000ml即可。0.02MPB（PH=7.4）：取100ml0.2MPB，加水稀釋至1000ml即可。?若需要NaCl的話，加入NaCl至0.9%（g/100ml）即可。另：其它各種另PH值的0.2MPB（100ml)配方：pH??????0.2MNaH2PO4（ml）????????0.2MNa2HPO4（ml）5.7???????????93.5?????????????????????????6.55.8?????????????92?????????????????????????????8????????????5.9????????????
4、90?????????????????????????????106.0????????????87.7?????????????????????????12.36.1????????????85????????????????????????????156.2?????????????81.5?????????????????????????18.56.3?????????????77.5?????????????????????????22.56.4????????????73.5?????????????????????????26.56.5????????????68.5???????
5、??????????????????31.56.6????????????62.5?????????????????????????37.56.7????????????56.5?????????????????????????43.56.8????????????51???????????????????????????496.9?????????????45???????????????????????????557.0?????????????38???????????????????????????627.1?????????????33????????????????????????
6、??677.2????????????28???????????????????????????727.3?????????????23???????????????????????????777.4?????????????19ml????????????????????????81ml7.5?????????????16???????????????????????????847.6?????????????13???????????????????????????877.7????????????10.5??????????????????????????90.57.8?????????
7、????8.5?????????????????????????91.57.9????????????7?????????????????????????????938.0????????????5.3?????????????????????????94.7?0.01M磷酸鹽緩沖液（PBS）配制方法稱取8gNaCl、0.2gKCl、1.44gNa2HPO4和0.24gKH2PO4,溶于800ml
