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1、咖啡因和維生素E對小尾寒羊精液冷凍保存的影響中國蓉學c亟握2010,26(8):l一6ChineseAgriculturalScienceBulletin咖啡因和維生素E對小尾寒羊精液冷凍保存的影響崔旭,羅桂河.,李衛(wèi)東.,李復煌,孟丹娜,呂建偉,穆笛,倪和民(北京農(nóng)學院動物科學技術系,北京102206;北京市昌平區(qū)動物衛(wèi)生監(jiān)督管理局,北京102200;北京市昌平區(qū)動物衛(wèi)生防疫站,北京102200;北京市畜牧獸醫(yī)總站,北京100107)摘要:研究冷凍稀釋液中分別添加不同濃度的咖啡因和維生素E后對小尾寒羊精液冷凍保存的影響.將不同濃度的咖啡因和維生素E分別添加于冷凍前的小尾寒羊精液稀釋液中,
2、用精子活率,頇體完整率和畸形率,作為判定精子冷凍品質(zhì)的指標,初步確定2種稀釋液添加成分對小尾寒羊精液品質(zhì)的影響.當添加維生素E的濃度為0.4mg/mL時,冷凍后的精子活率較高,畸形率較低,且精子頂體完整率也較高;添加5mg/mL咖啡因能顯著提高精子的解凍后活率,其活率為0.35,對精子畸形率和頂體完整率無顯著影響.在冷凍精液稀釋液中添加適當濃度維生素E可以減緩冷凍對精子的傷害,提高小尾寒羊冷凍精子活率,改善冷凍精液的品質(zhì);當添加5mg/mL的咖啡因于稀釋液中冷凍時,解凍后精液活率顯著提高,但不影響精子畸形率和頂體完整率.關鍵詞:咖啡因;維生素E;精液;小尾寒羊中圖分類號:S814.8文獻標
3、志碼:A論文編號:2009—2101EffectofDifferentConcentrationsofCaffeine,VEontheFreezingSemenofSmall—tailedHanSheepCuiXu,LuoGuihe,LiWeidong3,LiFuhuang4,MengDanna,LtiJianwei,MuDi,NiHeroin(DepartmentofAnimalScience&Technology,BengUniversityofAgriculture,Beijing102206;2AnimalHealthandHygieneAdministrationBure
4、auofBengChangpingDistrict,Beijing102200;3AnimalHealthandHygieneAdministrationStationofBeijingChangpingDistrict,Beijing102200;"Be~iingGeneralStationofAnimalHusbandryandVeterinaryMedicine,Beijing100107)Abstract:Thisstudywasaimedtoinvestigatetheeffectofdifferentconcentrationsofcaffeine,vitaminE(VE)on
5、thefreezingsemenofsmalltailedHansheep.ThedifferentconcentrationsofcaffeineandVEwereaddedtothepre-frozensheepsemendiluentinsmall-tailedHansheep,thenthemotilityrateoftheirpost-thawedse—men,thepercentageofspermswithintactacrosome,andthepercentageoftheirabnormality,wererespectivelyestimatedtodetectthe
6、qualityoftheirfrozensemen.Theresultswereshownasfollowing:Whiletheconcentra—tionofVEwas0.4mg/mL,afterfreezingandthawing,thelivabilityrateofthesemenfromsmalltailedHansheepishigh,whereastheirabnormalityrateislow,buttheapicalbodypercentageofspermheadisstillhigh.Meanwhile,addingwith5mg/mLcaffeineinthei
7、rfreezingdiluents,canalsoobviouslyimprovetheirspermliva—bilityrate(0.35),butnotaffecttheirabnormalrateandtheapicalbodyrate.AddingappropriateconcentrationsofvitaminEtothefrozensemendiluent,canslowthefreezingofsper