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1、七氟醚對脂多糖誘導(dǎo)的大鼠急性肺損傷的保護(hù)效應(yīng)【摘要】 目的:評估七氟醚后處理對脂多糖(LPS)致急性肺損傷(ALI)大鼠肺的影響。方法:隨機(jī)將30只大鼠分為5組(n=6):生理鹽水組、脂多糖組:氣管內(nèi)滴注生理鹽水1ml/kg或脂多糖5mg/kg;七氟醚30min組、七氟醚1h組、七氟醚2h組:氣管內(nèi)滴注脂多糖后4h,分別吸入2.4%七氟醚0.5,1,2h。各組于滴注生理鹽水或脂多糖后6h放血處死。檢測各組肺組織病理切片和肺泡灌洗液(BALF)中TNFα,IL1β和IL10的濃度。結(jié)果:與生理鹽水組比較,脂多糖組病理切片示肺泡正常結(jié)構(gòu)破壞嚴(yán)重,灌洗液中TNFα,IL
2、1β和IL10濃度顯著升高(P<0.01)。與脂多糖組比較,七氟醚組病理切片示肺泡結(jié)構(gòu)破壞較輕,灌洗液中TNFα,IL1β和IL10濃度降低(P<0.05);且1,2h組七氟醚改善病理形態(tài)和抑制TNFα,IL1β的效果更佳。結(jié)論:七氟醚后處理能減輕脂多糖致急性肺損傷大鼠肺部炎癥反應(yīng),且吸入時間長有更佳的效果?!娟P(guān)鍵詞】七氟醚;脂多糖;急性肺損傷;炎癥反應(yīng) ?。跘bstract]Objective:ToinvestigatetheeffectofsevofluranepostconditioningonlungsofratswithLPSinduced
3、acutelunginjury.Methods:Thirtyratswererandomlydividedinto5groups(n=6):NSgroup,LPSgroup,NS(1ml/kg)orLPS(59mg/kg)wasinstilledinrats′airway.Sevofluranegroups(S0.5hgroup,S1hgroup,S2hgroup):FourhoursafterLPSinstillation,ratsreceivedsevofluraneinhalation(2.4%)respectivelyforthirtyminutes,onehouro
4、rtwohours.SixhoursafterNSorLPSinstillation,allgroupswereexsanguinated.HistopathologicalexaminationswereperformedforthelungspecimensandconcentrationofTNFα,IL1βandIL10inBALFweremeasured.Results:ComparedwiththeNSgroup,afterLPSinstillation,thealveolarstructurewasseverelyaltered.Concentration
5、ofTNFα,IL1βandIL10inBALFweresignificantlyincreased(P<0.01).ComparedwiththeLPSgroup,insevofluranegroups,thealveolarstructurewasimproved.ConcentrationofTNFα,IL1βandIL10werereduced(P<0.05).ItwasmoreeffectivetoimprovepathohistologyandreduceTNFα,IL1βlevelsinS1hgroupandS2hgroupthanS
6、30mingroup.Conclusion:Sevofluranepostconditioningcouldattenuatelunginflammationinratswithlipopolysaccharideinducedacutelunginjury,itwasmoreeffectiveduringlongerinhalationtime. ?。跭eywords]sevoflurane;lipopolysaccharide;acutelunginjury;lunginflammation9 吸入麻醉藥物的抗炎效應(yīng)在體外實(shí)驗和一些器官系統(tǒng)(如心、腦、腎)中已經(jīng)得到了證
7、實(shí)[1-3]。然而吸入麻醉藥對肺損傷的保護(hù)作用,仍有待于系統(tǒng)的研究。本實(shí)驗予大鼠氣管內(nèi)滴注脂多糖復(fù)制急性肺損傷模型,觀察七氟醚后處理對大鼠肺泡灌洗液內(nèi)TNFα,IL1β和IL10的濃度及肺組織病理學(xué)的影響,為伴有急性肺損傷的高?;颊哌x用七氟醚麻醉提供動物實(shí)驗基礎(chǔ)?! ?材料與方法 1.1實(shí)驗動物及分組 健康清潔級雄性SD大鼠30只,體質(zhì)量220~250g。隨機(jī)分為5組,每組6只。生理鹽水組:氣管內(nèi)注入生理鹽水1ml/kg;脂多糖組:氣管內(nèi)注入脂多糖5mg/kg;七氟醚后處理組:氣管內(nèi)注入脂多糖