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1、吡咯烷二硫氨基甲酸酯對(duì)犬心肌線粒體損傷的保護(hù)作用作者：孟素峰，李有清，王啟明【摘要】目的觀察吡咯烷二硫氨基甲酸酯(PDTC)對(duì)體外循環(huán)(extracorporealcirculation,ECC)中犬心肌線粒體損傷的保護(hù)作用。方法12只犬隨機(jī)分為對(duì)照組(C組,n=6)和PDTC組(P組,n=6)，腹腔內(nèi)注射戊巴比妥鈉25mg/kg麻醉后建立ECC心肌缺血再灌注模型。P組于ECC前靜脈注射PDTC20mg/kg，C組靜脈注射等量生理鹽水。分別于ECC前、阻斷主動(dòng)脈60min、開放主動(dòng)脈30min和60min時(shí)，測(cè)定心肌線粒體超氧化物歧化酶（SOD）、谷胱甘肽過(guò)氧化
2、物酶(GSH-PX）、丙二醛(MDA)、總抗氧化能力(T-AOC)和線粒體腫脹度(MSD)。結(jié)果與ECC前比較，兩組阻斷主動(dòng)脈后SOD、GSH-PX及T-AOC均降低(P＜0.01)，MDA和MSD含量均升高；開放主動(dòng)脈后C組SOD、GSH-PX及T-AOC均降低(P＜0.01)，MSD和MDA含量均升高(P＜0.01)；P組在開放主動(dòng)脈60min時(shí)SOD、GSH-PX、T-AOC、MSD和MDA含量差異無(wú)統(tǒng)計(jì)學(xué)意義(P＞0.05)。P組在阻斷主動(dòng)脈60min時(shí)和開放主動(dòng)脈后SOD、GSH-PX及T-AOC均顯著高于C組(P＜0.01)，MSD和MDA含量均顯著
3、低于C組(P＜0.01)。結(jié)論ECC中使用PDTC可提高心肌線粒體抗氧化能力，減輕心肌線粒體損傷?！娟P(guān)鍵詞】10吡咯烷二硫氨基甲酸酯；線粒體；體外循環(huán)；心肌保護(hù)Abstract:OBJECTIVEToobservetheeffectofpyrrolidinedithiocarbamate(PDTC)oncaninemyocardialmitochondrialinjuryduringextracorporealcirculation(ECC).METHODSTwelvehealthymongreldogsundergoingECCwererandomlyallo
4、catedintocontrolgroup(groupC,n=6)andPDTCpretreatmentgroup(groupP,n=6).Alldogswereanesthesitizedwith2.5%sodiumpentobarbitone25mg/kg.Aftertrachealintubation,themyocardialischemia-reperfusioninjurymodelduringECCwasmadebydeclamping(DC)afteraorticcross-clamping(AC)for60min.IngroupP,PDTC20
5、mg/kgwasadministeredintravenouslybeforeECC，whileingroupCphysiologicalsalinewasgiveninsteadofPDTC.MyocardialmitochondriawereisolatedbeforeECC,60minafterAC，30minand60minafterDCfordeterminationofsuperoxidedismutase(SOD),glutathioneperoxidase(GSH-PX),malondialdehyde(MDA),totalanti-oxidat
6、ioncapacity(T-AOC)andmitochondrialswellingdegree(MSD).RESULTSThemyocardialmitochondriaofSOD,GSH-PXandT-AOCweredecreased，whileMDAandMSDwereincreasedafterACascomparedtothevaluebeforeECCinbothgroups(P＜0.01).SOD,GSH-PXandT-AOCweredecreased，whileMDAandMSDwereincreasedafterDC10ascomparedto
7、thevaluebeforeECCingroupC(P＜0.01).ThemyocardialmitochondriaofgroupPat60minafterDChadnotsignificantdifferencesincontentofMDA,SOD,GSH-PX,T-AOCandMSD(P＞0.05).SOD,GSH-PXandT-AOCweredistinctlylower，whileMDAandMSDweredistinctlyhigherat60minafterACandafterDCingroupPthanthoseingroupC(P＜0.01)
8、.CONCLUSIONP
