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1、遼寧中醫(yī)藥學(xué)報十五卷護骨膠囊對成骨細胞增殖、分化影響李寶紅1，吳勁東2，趙文昌1，宋麗軍1(1.廣東醫(yī)學(xué)院藥學(xué)院廣東東莞523808；廣東醫(yī)學(xué)院藥理學(xué)教研室，廣東東莞523808)摘要：目的：探討護骨膠囊對體外培養(yǎng)的大鼠成骨細胞增殖、分化的影響。方法：采用多次酶消化法分離出大鼠顱蓋骨的成骨細胞,采用MTT法和堿性磷酸酶法分別檢測護骨膠囊對成骨細胞增殖和分化的作用;采用SYBRgreenI嵌合的RT-PCR方法檢測Runx2mRNA表達水平。結(jié)果：護骨膠囊在濃度0.025~2.5μg/mL對成骨細胞有促增殖的作用,護骨膠囊在濃度0.025~25μg/mL對堿性磷酸酶的活性均有提高
2、的作用,護骨膠囊在濃度25μg/mL下,對Runx2mRNA水平有上調(diào)的作用。結(jié)論：護骨膠囊對成骨細胞有促增殖、促分化的作用,可能與上調(diào)Runx2mRNA水平有關(guān)。關(guān)鍵詞：護骨膠囊；成骨細胞；堿性磷酸酶；Runx2中圖分類號：R283文獻標志碼：A文章編號：1673-842X（2013）10-0024-03EffectofCapsuleonProliferationandDifferentiationofPrimaryOsteoblastsinVitroLIBaohong,WUJindong,ZHAOWenchang,SONGLjiun(1.CollegeofPharmacy,
3、GuangdongMedicalCollege,Dongguan523808,GuangdongChina;2.DepartmentofPharmacology,GuangdongMedicalCollege,Dongguan523808Guangdong,China)Abstract:Objective:ToinvestigatetheeffectofHuguCapsuleonproliferationanddifferentiationinprimaryosteoblasts.Methods:Bonecellswereobtainedbyenzymedigestionfro
4、mthesegregatedneonatalSDratskull.TheeffectsofHuguCapsuleontheproliferationanddifferentiationofratosteoblastswereevaluatedbytheMTTmethod,measuringtheavtivityofalkalinephosphatase,respectively.Runx2expressionwasanalyzedbyreal-timefluorescentquantitativereversetranscriptasepolymerasechainreacti
5、on(qRT-PCR)usingSYBAGreenI.Results:Withtheconcentrationof0.025-2.5μg/mL,HuguCapsulehadsignificanteffectontheROB’sproliferation.TheALPactivitywassignificantlyincreasedattheconcentrationfrom0.025μg/mLto25μg/mL.Withtheconcentrationof25μg/mL,HuguCapsulecanincreasetheRunx2MrnalocatedintheTomb,Don
6、gShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(WuzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,whennightcame遼寧中醫(yī)藥學(xué)報十五卷level.Conclusions:HuguCapsulecanstimulatetheproliferationanddifferentiationofosteoblastsinvitro,andthe
7、reasonmaybeconnectedwiththeupregulationofRunx2mRNAlevel.Keywords:HuguCapsule;osteoblasts;alkalinephosphate;Runx2護骨膠囊是由淫羊藿、制何首烏、巴戟天、骨碎補、龜甲、山藥、熟地黃、當歸、杜仲、續(xù)斷等組成的中藥復(fù)方新藥，臨床試驗表明其具有較好的治療原發(fā)性骨質(zhì)疏松癥和絕經(jīng)后骨質(zhì)疏松癥的作用[1-2]。但目前對其抗骨質(zhì)疏松的作用機制尚未進行深入研究，本項目通過探討護骨膠囊對大鼠原代成骨細胞
