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1、鱈魚皮膠原蛋白肽酶解液脫色脫腥工藝的研究郭玉華,李鈺金,吳新穎,邵仁東(泰祥集團;山東省海洋食品營養(yǎng)研究院,榮成264309)摘要:魚皮膠原蛋白肽是指魚皮膠原或明膠經蛋白酶等降解處理后制得的產物。該產物在氨基酸組成方面與大分子的膠原蛋白不相上下,而且更容易被人體吸收,還表現出超出大分子膠原蛋白的生理活性,可廣泛應用于食品、醫(yī)藥和保健品等行業(yè)。近年來,由于口蹄疫、瘋牛病等動物疾病的爆發(fā)及宗教等原因,從水產品中提取膠原蛋白或膠原蛋白多肽已成為國內外研究的熱點。但膠原蛋白水解產物具有令人不愉快的顏色和氣味,限制了其推廣應用。本研究以蛋白質損失率和感官值為
2、考察指標,通過單因素和正交實驗,研究了活性炭對水解產物脫色脫腥的效果。確定最佳脫色脫腥工藝條件為活性炭用量0.3%(w/v)、溫度50℃、時間45rain、pH5.5,在此條件下水解產物蛋白質損失率為4.2%,感觀值為8,基本達到了脫色脫腥的目的,提高了水解蛋白的品質。關鍵詞:鱈魚皮;膠原蛋白肽;酶解液;脫色;脫腥中圖分類號:TS202.1文獻標識碼:A文章編號:1006—2513(2010)04—0125—04Studyondecolorizinganddeodorizationofcodskinenzymolysiscollagenpeptid
3、eliquidGUOYu-hua,LIYu-jin,WUXin-ying,SHAORen-dong(GroupofTaixiang;FoodandNutritionResearchInstituteofShandongProvincemarine,Rongcheng264309)Abstract:Fishskincollagenpeptidereferstothedegradationofthecollagenorgelatinofthefishskinbytheprotease.Theaminoacidcompositionoftheproduc
4、tswerecomparablewiththecollagenmolecules,butweremoreeasilyabsorbedbythebody.Itwasalsodemonstratedthephysiologicalactivityofsupercollagenmolecules.Theycanbewidelyusedinfood,medicineandheahhcareproducts.Inrecentyears,duetotheoutbreakofanimaldiseasesuchasfootandmouthdisease,madCO
5、Wdiseases,aswellasreligiousandotherreasons,collagenorcollagenpeptideextractedfromaquaticproducthasbecomeahotresearchathomeandabroad.However,theyoftenhavetheunpleasantcolorandodorwhichlimiteditspopularizationandapplication.Inthispaper,thelossrateofproteinandsensoryvaluewasuseda
6、stheindexes,throughsingle—factortestandorthogonaltest,theefectofdecolorizinganddeodorizationoftheenzymol—ysisproductsbyactivatedcarbonwasinvestigated.Resuhfromthisstudyindicatedthatthebestconditionofdecolori—zinganddeodorizationwasactivatedcarbon0.3%(w/v),temperature50%,time45
7、min,pH5.5.Thelossratioofhydrolyzedproteinwas4.2%andthesensoryvaluewas8undertheoptimalconditions.Thequalityofhydrolyzedpro—teinwasimproved.Keywords:codskin;collagenpeptide;enzymolysisliquid;decolorization;deodorization收稿日期:2009—09—23作者簡介:郭玉華(1982一),女,碩士,研究方向:水產品高值化利用。生ChinaFood
8、Afff1,竺I‘堡=!魚膠原蛋白肽營養(yǎng)豐富,易于消化吸收,具液浸泡8~14h,過濾除去上清液,沉淀物用水有降m壓、降血脂